The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

John Day; Maike Lorenz; Tom Wilding; Thomas Friedl; Keith Harding; Thomas Pröschold; Debra Brennan; Julia Müller; Lilia Santos; Fatima Santos; Uugo Osorio; Raquel Amaral; Josef Elster; Jaromir Lukavský; Alena Lukesova; P Hrouzek; M Lukes; Ian Probert; M.J. Ryan; Erica E Benson

The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

John Day
, Maike Lorenz
, Tom Wilding
, Thomas Friedl
, Keith Harding
, Thomas Pröschold
, Debra Brennan
, Julia Müller
, Lilia Santos
, Fatima Santos
, Uugo Osorio
, Raquel Amaral
, Josef Elster
, Jaromir Lukavský
, Alena Lukesova
, P Hrouzek
, M Lukes
, Ian Probert
, M.J. Ryan
, Erica E Benson

科研成果: Article › 同行评审

21 引用（Scopus）

摘要

Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P

源语言	English
页（从-至）	359-376
页数	18
期刊	Cryoletters
卷	28
期	5
出版状态	Published - 2007

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Day, J., Lorenz, M., Wilding, T., Friedl, T., Harding, K., Pröschold, T., Brennan, D., Müller, J., Santos, L., Santos, F., Osorio, U., Amaral, R., Elster, J., Lukavský, J., Lukesova, A., Hrouzek, P., Lukes, M., Probert, I., Ryan, M. J., & Benson, E. E. (2007). The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections. Cryoletters, 28(5), 359-376.

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title = "The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections",

abstract = "Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15\% of the median viability level (94\%). Significant differences (P",

keywords = " RESOURCE, AUSTIN, Physiology, IMMOBILIZED CHLORELLA, EUKARYOTIC ALGAE, UNIVERSITY-OF-TEXAS, FRAGMENT LENGTH POLYMORPHISM, MICROALGAE, ENCAPSULATION-DEHYDRATION, CONSERVATION, Biology",

author = "John Day and Maike Lorenz and Tom Wilding and Thomas Friedl and Keith Harding and Thomas Pr{\"o}schold and Debra Brennan and Julia M{\"u}ller and Lilia Santos and Fatima Santos and Uugo Osorio and Raquel Amaral and Josef Elster and Jaromir Lukavsk{\'y} and Alena Lukesova and P Hrouzek and M Lukes and Ian Probert and M.J. Ryan and Benson, \{Erica E\}",

year = "2007",

language = "English",

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pages = "359--376",

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Day, J, Lorenz, M, Wilding, T, Friedl, T, Harding, K, Pröschold, T, Brennan, D, Müller, J, Santos, L, Santos, F, Osorio, U, Amaral, R, Elster, J, Lukavský, J, Lukesova, A, Hrouzek, P, Lukes, M, Probert, I, Ryan, MJ & Benson, EE 2007, 'The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections', Cryoletters, 卷 28, 号码 5, 页码 359-376.

TY - JOUR

T1 - The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

AU - Day, John

AU - Lorenz, Maike

AU - Wilding, Tom

AU - Friedl, Thomas

AU - Harding, Keith

AU - Pröschold, Thomas

AU - Brennan, Debra

AU - Müller, Julia

AU - Santos, Lilia

AU - Santos, Fatima

AU - Osorio, Uugo

AU - Amaral, Raquel

AU - Elster, Josef

AU - Lukavský, Jaromir

AU - Lukesova, Alena

AU - Hrouzek, P

AU - Lukes, M

AU - Probert, Ian

AU - Ryan, M.J.

AU - Benson, Erica E

PY - 2007

Y1 - 2007

N2 - Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P

AB - Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P

KW - RESOURCE

KW - AUSTIN

KW - Physiology

KW - IMMOBILIZED CHLORELLA

KW - EUKARYOTIC ALGAE

KW - UNIVERSITY-OF-TEXAS

KW - FRAGMENT LENGTH POLYMORPHISM

KW - MICROALGAE

KW - ENCAPSULATION-DEHYDRATION

KW - CONSERVATION

KW - Biology

M3 - Article

VL - 28

SP - 359

EP - 376

JO - Cryoletters

JF - Cryoletters

IS - 5

ER -

The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

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