@inbook{3a0e4df5fce44bd281247453a43ed188,
title = "Metabolomic analysis of Campylobacter jejuni by direct-injection electrospray ionization mass spectrometry",
abstract = "Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the high-throughput and relatively simple sample preparation of DIMS has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. Here we describe a metabolomic fingerprinting approach with sampling and extraction methodologies optimized for direct-injection electrospray ionization mass spectrometry (ESI-MS), which we have used as a means of comparing wild-type and isogenic mutant strains of C. jejuni with various metabolic blocks.",
keywords = "DIMS, Direct injection, Electrospray ionization mass spectrometry, Fingerprinting, Metabolite extraction, Metabolome, Metabolomics",
author = "Howlett, \{Robert M.\} and Davey, \{Matthew P.\} and Kelly, \{David J.\}",
note = "{\textcopyright} Springer Science+Business Media New York 2017 Produced while author was at the University of Sheffield",
year = "2017",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-6536-6\_16",
language = "English",
isbn = "978-1-4939-6534-2",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "189--197",
booktitle = "Methods in Molecular Biology",
}