An electrochemical method for detection of nucleic acid hybridisation

J M Hall; J Moore-Smith; J V Bannister; I J Higgins

An electrochemical method for detection of nucleic acid hybridisation

J M Hall
, J Moore-Smith
, J V Bannister
, I J Higgins

Centre for Rural Health Sciences

科研成果: Article › 同行评审

13 引用（Scopus）

摘要

Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.

源语言	English
页（从-至）	21-28
页数	8
期刊	Biochemistry and molecular biology international
卷	32
期	1
出版状态	Published - 1月 1994

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http://www.ncbi.nlm.nih.gov/pubmed/8012287?report=abstract

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abstract = "Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.",

keywords = "Animals, Biosensing Techniques, Calibration, Carbon, DNA, Complementary, DNA, Single-Stranded, Electrochemistry, Hydrogen-Ion Concentration, Nucleic Acid Hybridization",

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AB - Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.

KW - Animals

KW - Biosensing Techniques

KW - Calibration

KW - Carbon

KW - DNA, Complementary

KW - DNA, Single-Stranded

KW - Electrochemistry

KW - Hydrogen-Ion Concentration

KW - Nucleic Acid Hybridization

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An electrochemical method for detection of nucleic acid hybridisation

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