Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

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Original languageEnglish
Pages (from-to)1074-81
Number of pages8
JournalJournal of Fish Biology
Volume82
Issue number3
DOIs
StatePublished - 7 Mar 2013

    Research areas

  • Animals, DNA Damage, Hydrogen Peroxide/adverse effects, Male, Sperm Count, Spermatozoa/pathology, Zebrafish

Abstract

Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

Bibliographic note

© 2013 The Authors. Journal of Fish Biology © 2013 The Fisheries Society of the British Isles

ID: 3482285