An electrochemical method for detection of nucleic acid hybridisation

J M Hall; J Moore-Smith; J V Bannister; I J Higgins

An electrochemical method for detection of nucleic acid hybridisation

J M Hall
, J Moore-Smith
, J V Bannister
, I J Higgins

Centre for Rural Health Sciences

Résultats de recherche: Article › Revue par des pairs

13 Citations (Scopus)

Résumé

Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.

langue originale	English
Pages (de - à)	21-28
Nombre de pages	8
journal	Biochemistry and molecular biology international
Volume	32
Numéro de publication	1
état	Published - janv. 1994

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http://www.ncbi.nlm.nih.gov/pubmed/8012287?report=abstract

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title = "An electrochemical method for detection of nucleic acid hybridisation",

abstract = "Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.",

keywords = "Animals, Biosensing Techniques, Calibration, Carbon, DNA, Complementary, DNA, Single-Stranded, Electrochemistry, Hydrogen-Ion Concentration, Nucleic Acid Hybridization",

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year = "1994",

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journal = "Biochemistry and molecular biology international",

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AU - Hall, J M

AU - Moore-Smith, J

AU - Bannister, J V

AU - Higgins, I J

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N2 - Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.

AB - Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.

KW - Animals

KW - Biosensing Techniques

KW - Calibration

KW - Carbon

KW - DNA, Complementary

KW - DNA, Single-Stranded

KW - Electrochemistry

KW - Hydrogen-Ion Concentration

KW - Nucleic Acid Hybridization

M3 - Article

C2 - 8012287

SN - 1039-9712

VL - 32

SP - 21

EP - 28

JO - Biochemistry and molecular biology international

JF - Biochemistry and molecular biology international

IS - 1

ER -

An electrochemical method for detection of nucleic acid hybridisation

Résumé

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