Bell M.V., Dick, J.R. & Kelly, M.S. 2001. Biosynthesis of eicosapentaenoic acid in the sea urchin Psammechinus miliaris (Gmelin). Lipids 36 (1) 79 - 82

The sea urchin Psammechinus miliaris (Gmelin) (Echinodermata: Echinoidea) was shown by using a deuterated tracer (D-5-18:3n-3) and quantitation by negative chemical ionization gas chromatography-mass spectrometry to convert 18:3n-3 to 20:5n-3. The rate of conversion was very slow, corresponding to 0.09 mug/g tissue/mg 18:3n-3 eaten over 14 d. Deuterated arachidonic acid (D-8-20:4n-6) was also included in the diet to give a measure of the relative amounts of diet eaten by the different animals. The recovery of this fatty acid in tissue lipids was 33.7% compared with only 0.95% recovery of D-5 18:3n-3 and its anabolites, indicating that the majority of the D-5 tracer was catabolized. Considerable elongation of D-5-18:3n-3 into 20:3n-3 and a trace of 22:3n-3 was found, and these were accompanied by minor amounts of the intermediates 18:4n-3 and 20:4n-3. No deuterated 22:6n-3 was found.