Abstract
In this Study, We investigated the yield and physicochemical properties of the high molecular weight extracellular polymeric substance (HMW-EPS) produced by Halomonas sp. strain TG39 When grown on different types and ratios of substrates, Glucose (1% w/v) and a peptone/yeast extract ratio of 5.1 (0.6% w/v final concentration) yielded an EPS fraction (HMW-glucose) exhibiting the highest anionic activity (20.5) and specific emulsifying activity (EI24 = 100%) compared to EPS produced by cells grown on mannitol, sucrose, malt extract or no carbon source. The HMW-EPS fractions were capable of binding approximate to 255-464 mg of methylene blue (MB) per gram of EPS, which represents the highest reported binding of MB by a bacterial EPS. A comparative evaluation of these properties to those of commercial hydrocolloids indicated that the combined effect of protein and anionic residues of the HMW-EPS contributed to its ability to emulsify n-hexadecane. Liquid chromatography revealed the HMW-glucose EPS to be a heterogeneous polymer with a polydispersity index of 1.8. This work presents evidence of a correlation between the anionic nature and protein content of bacterial EPS with its emulsifying qualities, and identifies EPS produced by strain TG39 as a high MB-binding bacterial sorbant With potential biotechnological application.
Original language | English |
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Pages (from-to) | 207-216 |
Number of pages | 10 |
Journal | BIOTECHNOL BIOENG |
Volume | 103 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2009 |
Keywords
- BIOEMULSIFIERS
- METHANOLYSIS
- Biotechnology & Applied Microbiology
- URONIC-ACID
- MICROBIAL POLYSACCHARIDES
- HYDROCARBONS
- EURIHALINA
- EXTRACELLULAR POLYSACCHARIDES
- METHYLENE-BLUE
- EMULSIFYING ACTIVITY
- BACTERIAL EXOPOLYSACCHARIDES