Tissue proteomics in vascular disease

Amaya Albalat, William Mullen, Holger Husi, Harald Mischak

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

1 Citation (Scopus)

Abstract

In vascular diseases, as in many other pathophysiological conditions, tissue proteins are subjected to a number of different changes such as protein expression, posttranslational modifications, and proteolytic cleavage. For this reason, the study of the tissue proteome is becoming an increasingly important tool in biological and clinical research. In this chapter, we describe in detail the methodology for the analysis of tryptic digested peptides from aortic tissue extracts from mice with the aim to elucidate differences in the proteome between control and case or vascular diseased tissue samples. The method encompasses the analysis of these complex extracts in a single chromatographic run allowing for increased reproducibility. We also outline the main workflow for the processing of the generated data by statistical and bioinformatic analysis.
Original languageEnglish
Title of host publicationHypertension: Methods and Protocols
EditorsRhian M. Touyz, Ernesto L. Schiffrin
Place of PublicationNew York
PublisherSpringer New York
Pages53-60
Number of pages8
Volume1527
ISBN (Print)9781493966233
DOIs
Publication statusPublished - 24 Jan 2017

Publication series

NameMethods in molecular biology
PublisherSpringer New York

Fingerprint Dive into the research topics of 'Tissue proteomics in vascular disease'. Together they form a unique fingerprint.

  • Cite this

    Albalat, A., Mullen, W., Husi, H., & Mischak, H. (2017). Tissue proteomics in vascular disease. In R. M. Touyz, & E. L. Schiffrin (Eds.), Hypertension: Methods and Protocols (Vol. 1527, pp. 53-60). (Methods in molecular biology). Springer New York. https://doi.org/10.1007/978-1-4939-6625-7_4