The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

John Day, Maike Lorenz, Tom Wilding, Thomas Friedl, Keith Harding, Thomas Pröschold, Debra Brennan, Julia Müller, Lilia Santos, Fatima Santos, Uugo Osorio, Raquel Amaral, Josef Elster, Jaromir Lukavský, Alena Lukesova, P Hrouzek, M Lukes, Ian Probert, M.J. Ryan, Erica E Benson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P
Original languageEnglish
Pages (from-to)359-376
Number of pages18
JournalCryoletters
Volume28
Issue number5
Publication statusPublished - 2007

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Keywords

  • RESOURCE
  • AUSTIN
  • Physiology
  • IMMOBILIZED CHLORELLA
  • EUKARYOTIC ALGAE
  • UNIVERSITY-OF-TEXAS
  • FRAGMENT LENGTH POLYMORPHISM
  • MICROALGAE
  • ENCAPSULATION-DEHYDRATION
  • CONSERVATION
  • Biology

Cite this

Day, J., Lorenz, M., Wilding, T., Friedl, T., Harding, K., Pröschold, T., ... Benson, E. E. (2007). The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections. Cryoletters, 28(5), 359-376.