The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

John Day; Maike Lorenz; Tom Wilding; Thomas Friedl; Keith Harding; Thomas Pröschold; Debra Brennan; Julia Müller; Lilia Santos; Fatima Santos; Uugo Osorio; Raquel Amaral; Josef Elster; Jaromir Lukavský; Alena Lukesova; P Hrouzek; M Lukes; Ian Probert; M.J. Ryan; Erica E Benson

The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

John Day, Maike Lorenz, Tom Wilding, Thomas Friedl, Keith Harding, Thomas Pröschold, Debra Brennan, Julia Müller, Lilia Santos, Fatima Santos, Uugo Osorio, Raquel Amaral, Josef Elster, Jaromir Lukavský, Alena Lukesova, P Hrouzek, M Lukes, Ian Probert, M.J. Ryan, Erica E Benson

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P

Original language	English
Pages (from-to)	359-376
Number of pages	18
Journal	Cryoletters
Volume	28
Issue number	5
Publication status	Published - 2007

Keywords

RESOURCE
AUSTIN
Physiology
IMMOBILIZED CHLORELLA
EUKARYOTIC ALGAE
UNIVERSITY-OF-TEXAS
FRAGMENT LENGTH POLYMORPHISM
MICROALGAE
ENCAPSULATION-DEHYDRATION
CONSERVATION
Biology

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Day, J., Lorenz, M., Wilding, T., Friedl, T., Harding, K., Pröschold, T., Brennan, D., Müller, J., Santos, L., Santos, F., Osorio, U., Amaral, R., Elster, J., Lukavský, J., Lukesova, A., Hrouzek, P., Lukes, M., Probert, I., Ryan, M. J., & Benson, E. E. (2007). The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections. Cryoletters, 28(5), 359-376.

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title = "The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections",

abstract = "Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P",

keywords = " RESOURCE, AUSTIN, Physiology, IMMOBILIZED CHLORELLA, EUKARYOTIC ALGAE, UNIVERSITY-OF-TEXAS, FRAGMENT LENGTH POLYMORPHISM, MICROALGAE, ENCAPSULATION-DEHYDRATION, CONSERVATION, Biology",

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Day, J, Lorenz, M, Wilding, T, Friedl, T, Harding, K, Pröschold, T, Brennan, D, Müller, J, Santos, L, Santos, F, Osorio, U, Amaral, R, Elster, J, Lukavský, J, Lukesova, A, Hrouzek, P, Lukes, M, Probert, I, Ryan, MJ & Benson, EE 2007, 'The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections', Cryoletters, vol. 28, no. 5, pp. 359-376.

TY - JOUR

T1 - The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

AU - Day, John

AU - Lorenz, Maike

AU - Wilding, Tom

AU - Friedl, Thomas

AU - Harding, Keith

AU - Pröschold, Thomas

AU - Brennan, Debra

AU - Müller, Julia

AU - Santos, Lilia

AU - Santos, Fatima

AU - Osorio, Uugo

AU - Amaral, Raquel

AU - Elster, Josef

AU - Lukavský, Jaromir

AU - Lukesova, Alena

AU - Hrouzek, P

AU - Lukes, M

AU - Probert, Ian

AU - Ryan, M.J.

AU - Benson, Erica E

PY - 2007

Y1 - 2007

N2 - Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P

AB - Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P

KW - RESOURCE

KW - AUSTIN

KW - Physiology

KW - IMMOBILIZED CHLORELLA

KW - EUKARYOTIC ALGAE

KW - UNIVERSITY-OF-TEXAS

KW - FRAGMENT LENGTH POLYMORPHISM

KW - MICROALGAE

KW - ENCAPSULATION-DEHYDRATION

KW - CONSERVATION

KW - Biology

M3 - Article

VL - 28

SP - 359

EP - 376

JO - Cryoletters

JF - Cryoletters

IS - 5

ER -

The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

Abstract

Keywords

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