The antioxidant and chlorogenic acid profiles of whole coffee fruits are influenced by the extraction procedures

W. Mullen, B. Nemzer, B. Ou, A. Stalmach, J. Hunter, M. N. Clifford, E. Combet

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

Commercial whole coffee fruit extracts and powder samples were analyzed for chlorogenic acids (CGA), caffeine and antioxidant activities. CGA and caffeine were characterized by LC-MSn and HPLC accordingly, and quantified by UV absorbance. ORAC, HORAC, NORAC, SORAC and SOAC (antioxidant capacities) were assessed. Three caffeoylquinic acids, three feruloylquinic acids, three dicaffeoylquinic acids, one p-coumaroylquinic acid, two caffeoylferuloylquinic acids and three putative chlorogenic lactones were quantified, along with a methyl ester of 5-caffeoylquinic acid (detected in one sample, the first such report in any coffee material). Multistep whole coffee fruit extracts displayed higher CGA content than single-step extracts, freeze-dried, or air-dried whole raw fruits. Caffeine in multistep extracts was lower than in the single-step extracts and powders. Antioxidant activity in whole coffee fruit extracts was up to 25-fold higher than in powders dependent upon the radical. Total antioxidant activity of samples displayed strong correlation to CGA content.

Original languageEnglish
Pages (from-to)3754-3762
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number8
DOIs
Publication statusPublished - 27 Apr 2011

Keywords

  • antioxidant activity
  • caffeine
  • chlorogenic acid
  • coffee fruits
  • extract
  • green coffee

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