TY - JOUR
T1 - Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls
AU - Vukasinovic, Elvira L.
AU - Pond, David
AU - Grubor-Lajsic, Gordana
AU - Worland, Michael Roger
AU - Kojic, Danijela
AU - Purac, Jelena
AU - Jovanovic, Zeljko
AU - Blagojevic, Dusko P.
N1 - © Springer-Verlag Berlin Heidelberg 2017
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to -3°C and -10°C in mid-diapause (MD) when it significantly increased. Differential Scanning Calorimetry (DSC) was used to examine phase transitions of total body lipid of cold acclimated larvae in diapasue. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.
AB - Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to -3°C and -10°C in mid-diapause (MD) when it significantly increased. Differential Scanning Calorimetry (DSC) was used to examine phase transitions of total body lipid of cold acclimated larvae in diapasue. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.
KW - Cold acclimation
KW - Fatty acid composition Insect; Melt transition temperatures; Phospholipid;
KW - Insect
KW - Melt transition temperatures
KW - Phospholipid
KW - Triacylglycerol
U2 - 10.1007/s00360-017-1110-9
DO - 10.1007/s00360-017-1110-9
M3 - Article
SN - 0174-1578
SP - 1
EP - 10
JO - Journal of Comparative Physiology B- Biochemical Systemic and Environmental Physiology
JF - Journal of Comparative Physiology B- Biochemical Systemic and Environmental Physiology
ER -