TaqMan DNA technology confirms likely overestimation of cod (Gadus morhua L.) egg abundance in the Irish Sea: implications for the assessment of the cod stock and mapping of spawning areas using egg-based methods

Clive Fox, M I Taylor, R Pereyra, M I Villasana, C Rico

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Recent substantial declines in northeastern Atlantic cod stocks necessitate improved biological knowledge and the development of techniques to complement standard stock assessment methods (which largely depend on accurate commercial catch data). In 2003, an ichthyoplankton survey was undertaken in the Irish Sea and subsamples of 'cod-like' eggs were analysed using a TaqMan multiplex, PCR (polymerase chain reaction) assay (with specific probes for cod, haddock and whiting). The TaqMan method was readily applied to the large number of samples (n = 2770) generated during the survey and when combined with a manual DNA extraction protocol had a low failure rate of 6%. Of the early stage 'cod-like' eggs (1.2-1.75 mm diameter) positively identified: 34% were cod, 8% haddock and 58% whiting. As previous stock estimates based on egg surveys for Irish Sea cod assumed that the majority of 'cod-like' eggs were from cod, the TaqMan results confirm that there was probably substantial contamination by eggs of whiting and haddock that would have inflated estimates of the stock biomass.
Original languageEnglish
Pages (from-to)879-884
Number of pages6
JournalMOL ECOL
Volume14
Issue number3
DOIs
Publication statusPublished - 2005

    Fingerprint

Keywords

  • MITOCHONDRIAL-DNA
  • Ecology
  • Biochemistry & Molecular Biology
  • AMPLIFICATION
  • EXTRACTION
  • Evolutionary Biology
  • IDENTIFICATION

Cite this