Tailoring of the biochemical profiles of microalgae by employing mixotrophic cultivation

Penhaul Smith J.K., Hughes A.D., McEvoy L., Day J.G.

Research output: Contribution to journalArticle

Abstract

Mixotrophy, in marine microalgae, is the ability to uptake organic nutrients from the environment, in addition to, or as an alternative to, fixation of carbon through photosynthesis. Many microalgae are capable of mixotrophic and heterotrophic growth, which can be used to maximise culture density, while theoretically lowering production costs compared to photoautotrophy. Here we show that, following screening of three microalgal species for mixotrophic and heterotrophic growth, Tetraselmis suecica and Cyclotella cryptica were capable of mixotrophic growth on glycerol, glucose and acetate and heterotrophically on glucose. Phaeodactylum tricornutum could only be cultured mixotrophically, but when cultured on glycerol cell density increased fourfold compared to photoautotrophic culture. Carbon allocation changed dependent upon carbon source and time of harvesting, with mixotrophic culture of P. tricornutum on glycerol resulting in lower cell length and increased total fatty acid content. These changes can be utilised in the development of a ‘designer alga’.
Original languageEnglish
Article number100321
Number of pages11
JournalBioresource Technology Reports
Volume9
Early online date7 Nov 2019
DOIs
Publication statusPublished - Feb 2020

Fingerprint

microalgae
glycerol
Phaeodactylum tricornutum
algae
Tetraselmis suecica
Cyclotella
glucose
Calvin cycle
carbon
autotrophs
production costs
fatty acid composition
acetates
photosynthesis
cells
screening
uptake mechanisms
nutrients

Keywords

  • ‘designer algae’
  • mixotrophy
  • aquaculture
  • biotechnology

Cite this

@article{c12311eff189443ab94694419ca754db,
title = "Tailoring of the biochemical profiles of microalgae by employing mixotrophic cultivation",
abstract = "Mixotrophy, in marine microalgae, is the ability to uptake organic nutrients from the environment, in addition to, or as an alternative to, fixation of carbon through photosynthesis. Many microalgae are capable of mixotrophic and heterotrophic growth, which can be used to maximise culture density, while theoretically lowering production costs compared to photoautotrophy. Here we show that, following screening of three microalgal species for mixotrophic and heterotrophic growth, Tetraselmis suecica and Cyclotella cryptica were capable of mixotrophic growth on glycerol, glucose and acetate and heterotrophically on glucose. Phaeodactylum tricornutum could only be cultured mixotrophically, but when cultured on glycerol cell density increased fourfold compared to photoautotrophic culture. Carbon allocation changed dependent upon carbon source and time of harvesting, with mixotrophic culture of P. tricornutum on glycerol resulting in lower cell length and increased total fatty acid content. These changes can be utilised in the development of a ‘designer alga’.",
keywords = "‘designer algae’, mixotrophy, aquaculture, biotechnology",
author = "J.K., {Penhaul Smith} and Hughes A.D. and McEvoy L. and Day J.G.",
note = "{\circledC} 2019 Elsevier Ltd. All rights reserved.",
year = "2020",
month = "2",
doi = "10.1016/j.biteb.2019.100321",
language = "English",
volume = "9",
journal = "Bioresource Technology Reports",
issn = "2589-014X",
publisher = "Elsevier",

}

Tailoring of the biochemical profiles of microalgae by employing mixotrophic cultivation. / J.K., Penhaul Smith; A.D., Hughes; L., McEvoy; J.G., Day.

In: Bioresource Technology Reports, Vol. 9, 100321, 02.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tailoring of the biochemical profiles of microalgae by employing mixotrophic cultivation

AU - J.K., Penhaul Smith

AU - A.D., Hughes

AU - L., McEvoy

AU - J.G., Day

N1 - © 2019 Elsevier Ltd. All rights reserved.

PY - 2020/2

Y1 - 2020/2

N2 - Mixotrophy, in marine microalgae, is the ability to uptake organic nutrients from the environment, in addition to, or as an alternative to, fixation of carbon through photosynthesis. Many microalgae are capable of mixotrophic and heterotrophic growth, which can be used to maximise culture density, while theoretically lowering production costs compared to photoautotrophy. Here we show that, following screening of three microalgal species for mixotrophic and heterotrophic growth, Tetraselmis suecica and Cyclotella cryptica were capable of mixotrophic growth on glycerol, glucose and acetate and heterotrophically on glucose. Phaeodactylum tricornutum could only be cultured mixotrophically, but when cultured on glycerol cell density increased fourfold compared to photoautotrophic culture. Carbon allocation changed dependent upon carbon source and time of harvesting, with mixotrophic culture of P. tricornutum on glycerol resulting in lower cell length and increased total fatty acid content. These changes can be utilised in the development of a ‘designer alga’.

AB - Mixotrophy, in marine microalgae, is the ability to uptake organic nutrients from the environment, in addition to, or as an alternative to, fixation of carbon through photosynthesis. Many microalgae are capable of mixotrophic and heterotrophic growth, which can be used to maximise culture density, while theoretically lowering production costs compared to photoautotrophy. Here we show that, following screening of three microalgal species for mixotrophic and heterotrophic growth, Tetraselmis suecica and Cyclotella cryptica were capable of mixotrophic growth on glycerol, glucose and acetate and heterotrophically on glucose. Phaeodactylum tricornutum could only be cultured mixotrophically, but when cultured on glycerol cell density increased fourfold compared to photoautotrophic culture. Carbon allocation changed dependent upon carbon source and time of harvesting, with mixotrophic culture of P. tricornutum on glycerol resulting in lower cell length and increased total fatty acid content. These changes can be utilised in the development of a ‘designer alga’.

KW - ‘designer algae’

KW - mixotrophy

KW - aquaculture

KW - biotechnology

U2 - 10.1016/j.biteb.2019.100321

DO - 10.1016/j.biteb.2019.100321

M3 - Article

VL - 9

JO - Bioresource Technology Reports

JF - Bioresource Technology Reports

SN - 2589-014X

M1 - 100321

ER -