Size-fractionated carboxylase activities during a 32 h cycle at 30 m depth in the Northwestern Mediterranean Sea after an episodic wind event

Eric Fouilland, Claude Courties, Chantal Descolas-Gros

Research output: Contribution to journalArticle

Abstract

Using size fractionation filtration (1 mum), we associated carboxylase activities (Rubisco, beta -carboxylases) and chlorophyll measurements with cell enumeration by flow cytometry at a permanent site of the central Ligurian,Sea in the north-western Mediterranean Sea (73 degrees 25 'N-7 degrees 51 'E). The analyses were carried out over a day/night Cycle (at 30 m depth) following a strong wind event, during the transition period from spring mesotrophic to summer oligotrophic conditions. The highest values of Rubisco activity and beta -carboxylase activity per chlorophyll a (Chl a) for >1 mum cells were observed during the light period of the cycle, reaching 18.9 and 4.3 nmol CO2 (mug Chl a)(-1) h(-1), respectively. This higher activity is assumed to be correlated with a dominance of nanoflagellates in the phytoplankton community. Such phytoplankton species generally had higher beta -carboxylase activity, expressed as a percentage of Rubisco activity (the betaC/R ratio), than diatoms. Using ooze, cytometry analysis to enumerate those cells
Original languageEnglish
Pages (from-to)623-632
Number of pages10
JournalJ PLANKTON RES
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • CARBON FIXATION
  • MARINE-PHYTOPLANKTON
  • PHOTOSYNTHESIS
  • BETA-CARBOXYLATION
  • METABOLIC-ACTIVITY
  • DIATOMS
  • Marine & Freshwater Biology
  • Oceanography
  • PICOPLANKTON
  • GROWTH-RATES
  • FLOW-CYTOMETRY
  • CELL

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