Regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (Cyprinus carpio)

Philip Brownridge, Luciane Vieira de Mello, Mary Peters, Lynn McLean, Amy Claydon, Andrew R Cossins, Iain S Young, Phil Whitfield

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The mechanical properties of the axial muscles vary along the length of a fish's body. This variation in performance correlates with the expression of certain muscle proteins. Parvalbumin (PARV) is an important calcium binding protein that helps modulate intracellular calcium levels which set the size and shape of the muscle calcium transient. It therefore has a central role in determining the functional properties of the muscle. Transcript data revealed eight specific isoforms of PARV in common carp (Cyprinus carpio) skeletal muscle which we classified as alpha1 and beta1-7. This study is the first to show expression of all eight skeletal muscle PARV isoforms in carp at the protein level and relate regional differences in expression to performance. All of the PARV isoforms were characterised at the protein level using 2D-PAGE and tandem mass spectrometry. Comparison of carp muscle from different regions of the fish revealed a higher level of expression of PARV isoforms beta4 and beta5 in the anterior region, which was accompanied by an increase in the rate of relaxation. We postulate that changes in specific PARV isoform expression are an important part of the adaptive change in muscle mechanical properties in response to varying functional demands and environmental change.
Original languageEnglish
Pages (from-to)184-93
Number of pages10
JournalJournal of Experimental Biology
Volume212
Issue numberPt 2
DOIs
Publication statusPublished - Jan 2009

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Keywords

  • Animals
  • Biomechanics
  • Carps
  • Gene Expression Profiling
  • Muscle Fibers, Fast-Twitch
  • Parvalbumins
  • Protein Isoforms

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