Regional mtDNA SNP differentiation in European Atlantic salmon (Salmo salar): an assessment of potential utility for determination of natal origin

Eric Verspoor, Sonia Consuegra, Olafur Fridjonsson, Sigridur Hjorleifsdottir, David Knox, Kristinn Olafsson, Scott Tompsett, Vidar Wennevik, Carlos Garcia de Leaniz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Atlantic salmon, Salmo salar, shows geographically structured differentiation at various classes of molecular genetic variation, among and within river stocks. Nuclear microsatellite locus variation at multiple loci has been exploited for more than a decade as a marker for the continental origin of fish caught at sea in distant-water fisheries. However, a simpler, more cost-effective, but still accurate, assignment can be obtained using a single microsatellite locus in combination with a mitochondrial DNA (mtDNA) single-nucleotide polymorphism (SNP) detected by restriction enzyme digestion. Following on from this, a preliminary study was made of the potential for using mtDNA SNP variation to enhance the resolving power and cost-effectiveness of within-continent assignment of European salmon as determined using microsatellites. Variation in 20 mtDNA regions, encompassing ∼43% of this genome, in 330 salmon from 29 rivers across Europe, was analysed. High levels of inter-individual and inter-river variation were found, as well as evidence of regional differentiation paralleling observed microsatellite differentiation. The observations indicate scope for using mtDNA SNPs along with microsatellites for genetically based assignment of European salmon to region and river of natal origin, but further study is needed.
Original languageEnglish
Pages (from-to)1625-1636
Number of pages11
JournalICES Journal of Marine Science
Volume69
Issue number9
DOIs
Publication statusPublished - 20 Mar 2012

Keywords

  • Atlantic salmon
  • genetic stock identification
  • marine ecology
  • mitochondrial DNA
  • 454 sequencing

Cite this

Verspoor, E., Consuegra, S., Fridjonsson, O., Hjorleifsdottir, S., Knox, D., Olafsson, K., Tompsett, S., Wennevik, V., & de Leaniz, C. G. (2012). Regional mtDNA SNP differentiation in European Atlantic salmon (Salmo salar): an assessment of potential utility for determination of natal origin. ICES Journal of Marine Science, 69(9), 1625-1636. https://doi.org/10.1093/icesjms/fss029