Reduced pH increases mortality and genotoxicity in an Arctic coastal copepod, Acartia longiremis

Claudia Halsband; Mascha F Dix; Kristine Hopland Sperre; Helena C Reinardy

doi:10.1016/j.aquatox.2021.105961

Reduced pH increases mortality and genotoxicity in an Arctic coastal copepod, Acartia longiremis

Claudia Halsband, Mascha F Dix, Kristine Hopland Sperre, Helena C Reinardy

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Abstract

This study investigates DNA damage and mortality in an Arctic marine copepod after long-term exposure to lowered pH. Acartia longiremis were collected from northern Norway and incubated in ambient pH 8.1, and reduced pH 7.6 and 7.2 over 3-4 weeks. Cumulative mortality was significantly elevated in the lowered pH treatments in all exposures. The fluorescence-based fast micromethod for analysis of DNA strand breaks and alkali-labile sites was modified for use on crustaceous zooplankton. DNA damage initially increased in the lowered pH treatments, decreasing after >14 days, and DNA damage was significantly higher in lowered pH conditions. This method is ideal for investigating oxidative stress and genotoxicity response to low pH in Arctic marine copepods exposed to future ocean acidification conditions.

Original language	English
Article number	105961
Number of pages	4
Journal	Aquatic Toxicology
Volume	239
DOIs	https://doi.org/10.1016/j.aquatox.2021.105961
Publication status	Published - 4 Sept 2021

Keywords

DNA Damage
fast micromethod
oxidative stress
zooplankton
Crustacea

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.aquatox.2021.105961Licence: CC BY

1-s2.0-S0166445X21002204-mainFinal published version, 493 KBLicence: CC BY

Cite this

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title = "Reduced pH increases mortality and genotoxicity in an Arctic coastal copepod, Acartia longiremis",

abstract = "This study investigates DNA damage and mortality in an Arctic marine copepod after long-term exposure to lowered pH. Acartia longiremis were collected from northern Norway and incubated in ambient pH 8.1, and reduced pH 7.6 and 7.2 over 3-4 weeks. Cumulative mortality was significantly elevated in the lowered pH treatments in all exposures. The fluorescence-based fast micromethod for analysis of DNA strand breaks and alkali-labile sites was modified for use on crustaceous zooplankton. DNA damage initially increased in the lowered pH treatments, decreasing after >14 days, and DNA damage was significantly higher in lowered pH conditions. This method is ideal for investigating oxidative stress and genotoxicity response to low pH in Arctic marine copepods exposed to future ocean acidification conditions.",

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author = "Claudia Halsband and Dix, {Mascha F} and Sperre, {Kristine Hopland} and Reinardy, {Helena C}",

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AU - Halsband, Claudia

AU - Dix, Mascha F

AU - Sperre, Kristine Hopland

AU - Reinardy, Helena C

PY - 2021/9/4

Y1 - 2021/9/4

N2 - This study investigates DNA damage and mortality in an Arctic marine copepod after long-term exposure to lowered pH. Acartia longiremis were collected from northern Norway and incubated in ambient pH 8.1, and reduced pH 7.6 and 7.2 over 3-4 weeks. Cumulative mortality was significantly elevated in the lowered pH treatments in all exposures. The fluorescence-based fast micromethod for analysis of DNA strand breaks and alkali-labile sites was modified for use on crustaceous zooplankton. DNA damage initially increased in the lowered pH treatments, decreasing after >14 days, and DNA damage was significantly higher in lowered pH conditions. This method is ideal for investigating oxidative stress and genotoxicity response to low pH in Arctic marine copepods exposed to future ocean acidification conditions.

AB - This study investigates DNA damage and mortality in an Arctic marine copepod after long-term exposure to lowered pH. Acartia longiremis were collected from northern Norway and incubated in ambient pH 8.1, and reduced pH 7.6 and 7.2 over 3-4 weeks. Cumulative mortality was significantly elevated in the lowered pH treatments in all exposures. The fluorescence-based fast micromethod for analysis of DNA strand breaks and alkali-labile sites was modified for use on crustaceous zooplankton. DNA damage initially increased in the lowered pH treatments, decreasing after >14 days, and DNA damage was significantly higher in lowered pH conditions. This method is ideal for investigating oxidative stress and genotoxicity response to low pH in Arctic marine copepods exposed to future ocean acidification conditions.

KW - DNA Damage

KW - fast micromethod

KW - oxidative stress

KW - zooplankton

KW - Crustacea

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DO - 10.1016/j.aquatox.2021.105961

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JO - Aquatic Toxicology

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M1 - 105961

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Reduced pH increases mortality and genotoxicity in an Arctic coastal copepod, Acartia longiremis

Abstract

Keywords

UN SDGs

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