Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples: comparison with other PCR techniques

Dolors Vidal; Mark A Taggart; Ignacio Badiola; Rafael Mateo

doi:10.1177/1040638711416847

Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples: comparison with other PCR techniques

Dolors Vidal, Mark A Taggart, Ignacio Badiola, Rafael Mateo

Environmental Research Institute

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A quantitative real-time PCR (qPCR) for detection of the neurotoxin of the Clostridium botulinum type C (BoNTC) encoding gene has been compared with a nested PCR (nPCR) and a conventional PCR (cPCR) using 2 toxigenic C. botulinum C1 reference strains and samples from bird tissues (n = 30) and sediments (n = 30) from wetlands where botulism outbreaks have been reported. A cPCR based on 16S ribosomal RNA sequences from 60 strains of Clostridium species was also developed to detect the genomic DNA of C. botulinum C in order to evaluate the presence of nontoxigenic strains. Quantitative PCR showed a similar sensitivity to nPCR (

Original language	English
Pages (from-to)	942-6
Number of pages	5
Journal	Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Volume	23
Issue number	5
DOIs	https://doi.org/10.1177/1040638711416847
Publication status	Published - 2011

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Vidal, D., Taggart, M. A., Badiola, I., & Mateo, R. (2011). Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples: comparison with other PCR techniques. Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 23(5), 942-6. https://doi.org/10.1177/1040638711416847

Vidal, Dolors ; Taggart, Mark A ; Badiola, Ignacio et al. / Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples : comparison with other PCR techniques. In: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 2011 ; Vol. 23, No. 5. pp. 942-6.

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abstract = "A quantitative real-time PCR (qPCR) for detection of the neurotoxin of the Clostridium botulinum type C (BoNTC) encoding gene has been compared with a nested PCR (nPCR) and a conventional PCR (cPCR) using 2 toxigenic C. botulinum C1 reference strains and samples from bird tissues (n = 30) and sediments (n = 30) from wetlands where botulism outbreaks have been reported. A cPCR based on 16S ribosomal RNA sequences from 60 strains of Clostridium species was also developed to detect the genomic DNA of C. botulinum C in order to evaluate the presence of nontoxigenic strains. Quantitative PCR showed a similar sensitivity to nPCR (",

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Vidal, D, Taggart, MA, Badiola, I & Mateo, R 2011, 'Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples: comparison with other PCR techniques', Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, vol. 23, no. 5, pp. 942-6. https://doi.org/10.1177/1040638711416847

Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples: comparison with other PCR techniques. / Vidal, Dolors; Taggart, Mark A; Badiola, Ignacio et al.
In: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Vol. 23, No. 5, 2011, p. 942-6.

Research output: Contribution to journal › Article › peer-review

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Vidal D, Taggart MA, Badiola I, Mateo R. Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples: comparison with other PCR techniques. Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 2011;23(5):942-6. doi: 10.1177/1040638711416847

Real-time polymerase chain reaction for the detection of toxigenic Clostridium botulinum type C1 in waterbird and sediment samples: comparison with other PCR techniques

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