Real-time PCR: what relevance to plant studies?" Journal of Experimental Botany 55(402): 1445-1454.

Claire Gachon; Annaick Mingam; Benedicte Charrier

doi:10.1093/jxb/erh181

Real-time PCR: what relevance to plant studies?" Journal of Experimental Botany 55(402): 1445-1454.

Claire Gachon, Annaick Mingam, Benedicte Charrier

SAMS UHI

Research output: Contribution to journal › Article › peer-review

390 Citations (Scopus)

Abstract

The appearance of genetically modified organisms on the food market a few years ago, and the demand for more precise and reliable techniques to detect foreign (transgenic or pathogenic) DNA in edible plants, have been the driving force for the introduction of real-time PCR techniques in plant research. This was followed by numerous fundamental research applications aiming to study the expression profiles of endogenous genes and multigene families. Since then, the interest in this technique in the plant scientist community has increased exponentially. This review describes the technical features of quantitative real-time PCR that are especially relevant to plant research, and summarizes its present and future applications.

Original language	English
Pages (from-to)	1445-1454
Number of pages	10
Journal	J EXP BOT
Issue number	5
DOIs	https://doi.org/10.1093/jxb/erh181
Publication status	Published - 2004

Keywords

ARABIDOPSIS-THALIANA
GENE-EXPRESSION
DEPENDENT EXPRESSION
MOLECULAR CHARACTERIZATION
RT-PCR
POLYMERASE-CHAIN-REACTION
QUANTITATIVE PCR
ALTERNARIA-BRASSICICOLA
GENETICALLY-MODIFIED ORGANISMS
MICROARRAY ANALYSIS
Plant Sciences

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title = "Real-time PCR: what relevance to plant studies?{"} Journal of Experimental Botany 55(402): 1445-1454.",

abstract = "The appearance of genetically modified organisms on the food market a few years ago, and the demand for more precise and reliable techniques to detect foreign (transgenic or pathogenic) DNA in edible plants, have been the driving force for the introduction of real-time PCR techniques in plant research. This was followed by numerous fundamental research applications aiming to study the expression profiles of endogenous genes and multigene families. Since then, the interest in this technique in the plant scientist community has increased exponentially. This review describes the technical features of quantitative real-time PCR that are especially relevant to plant research, and summarizes its present and future applications.",

keywords = " ARABIDOPSIS-THALIANA, GENE-EXPRESSION, DEPENDENT EXPRESSION, MOLECULAR CHARACTERIZATION, RT-PCR, POLYMERASE-CHAIN-REACTION, QUANTITATIVE PCR, ALTERNARIA-BRASSICICOLA, GENETICALLY-MODIFIED ORGANISMS, MICROARRAY ANALYSIS, Plant Sciences",

author = "Claire Gachon and Annaick Mingam and Benedicte Charrier",

year = "2004",

doi = "10.1093/jxb/erh181",

language = "English",

pages = "1445--1454",

journal = "J EXP BOT",

publisher = "Oxford University Press",

number = "5",

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TY - JOUR

T1 - Real-time PCR

T2 - what relevance to plant studies?" Journal of Experimental Botany 55(402): 1445-1454.

AU - Gachon, Claire

AU - Mingam, Annaick

AU - Charrier, Benedicte

PY - 2004

Y1 - 2004

N2 - The appearance of genetically modified organisms on the food market a few years ago, and the demand for more precise and reliable techniques to detect foreign (transgenic or pathogenic) DNA in edible plants, have been the driving force for the introduction of real-time PCR techniques in plant research. This was followed by numerous fundamental research applications aiming to study the expression profiles of endogenous genes and multigene families. Since then, the interest in this technique in the plant scientist community has increased exponentially. This review describes the technical features of quantitative real-time PCR that are especially relevant to plant research, and summarizes its present and future applications.

AB - The appearance of genetically modified organisms on the food market a few years ago, and the demand for more precise and reliable techniques to detect foreign (transgenic or pathogenic) DNA in edible plants, have been the driving force for the introduction of real-time PCR techniques in plant research. This was followed by numerous fundamental research applications aiming to study the expression profiles of endogenous genes and multigene families. Since then, the interest in this technique in the plant scientist community has increased exponentially. This review describes the technical features of quantitative real-time PCR that are especially relevant to plant research, and summarizes its present and future applications.

KW - ARABIDOPSIS-THALIANA

KW - GENE-EXPRESSION

KW - DEPENDENT EXPRESSION

KW - MOLECULAR CHARACTERIZATION

KW - RT-PCR

KW - POLYMERASE-CHAIN-REACTION

KW - QUANTITATIVE PCR

KW - ALTERNARIA-BRASSICICOLA

KW - GENETICALLY-MODIFIED ORGANISMS

KW - MICROARRAY ANALYSIS

KW - Plant Sciences

U2 - 10.1093/jxb/erh181

DO - 10.1093/jxb/erh181

M3 - Article

SP - 1445

EP - 1454

JO - J EXP BOT

JF - J EXP BOT

IS - 5

ER -

Real-time PCR: what relevance to plant studies?" Journal of Experimental Botany 55(402): 1445-1454.

Abstract

Keywords

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