Proteomic analysis of urinary upper gastrointestinal cancer markers

Holger Husi, Nathan Stephens, Andrew Cronshaw, Alisdair MacDonald, Iain Gallagher, Carolyn Greig, Kenneth C.H. Fearon, James A. Ross

Research output: Contribution to journalArticle

22 Citations (Scopus)


Purpose: We have investigated the use of human urine as a non-invasive medium to screen for molecular biomarkers of carcinomas of the upper gastrointestinal (uGI) tract using SELDI-TOF-MS.

Experimental design: A total of 120 urine specimens from 60 control and 60 uGI cancer patients were analysed to establish a potential biomarker fingerprint for the weak cation exchanger CM10 chip surface, which was validated by blind testing using a further 59 samples from 33 control and 26 uGI cancer patients.

Results: Using Biomarker Pattern software, we established a model with a sensitivity of 98% and specificity of 95% for the learning sample set, and a sensitivity of 96% and specificity of 72% for the validation data set. Model variable importance included six peptides with m/z of 10 230, 10 436, 10 574, 10 311, 10 467, and 1 0118 of which the 10 230 molecular species was the main decider (sensitivity 86% and specificity 80. Initial protein database searching identified 10 230 as S100-A6, 10 436 as S100-P, 10 467 as S100-A9, and 10 574 as S100-A12 of which S100-A6 and S100-A9 were confirmed by Western blotting.

Conclusions and clinical relevance: We have demonstrated that SELDI-TOF-MS as a screening tool is a rapid and valid methodology in the search for urinary cancer biomarkers, and is potentially useful in defining and consolidating biomarker patterns for uGI cancer screening.
Original languageEnglish
Pages (from-to)289-299
Number of pages11
JournalProteomics Clinical Applications
Issue number5-6
Publication statusPublished - 1 Jun 2011


Cite this

Husi, H., Stephens, N., Cronshaw, A., MacDonald, A., Gallagher, I., Greig, C., ... Ross, J. A. (2011). Proteomic analysis of urinary upper gastrointestinal cancer markers. Proteomics Clinical Applications, 5(5-6), 289-299.