The surface mucus layer (SML) is of critical importance in health, disease, and stress responses of corals; however, visualising the intact SML is challenging. Dehydration during histological preparation causes shrinkage and deformation of the mucus gel layer, while fragile, unattached mucus exudates are typically lost altogether. Here, we describe a novel technique using water-soluble glycol methacrylate resin embedding that more accurately preserves the in situ SML. Thickness of the preserved SML is similar to that previously measured on live corals using a glass microprobe. The technique allows microscopic visualisation of the SML structure, as well as thickness and continuity measurements, which are important indicators of SML function in health and disease.
|Number of pages||6|
|Early online date||23 Mar 2019|
|Publication status||Published - 15 Jun 2019|
- Coral SML
- Coral histology
- Preservation of mucus
- GMA resin
- Coral health
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- Environmental Research Institute - Associate Lecturer
Person: Academic Research Active