Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides

Robert J Beynon; Mary K Doherty; Julie M Pratt; Simon J Gaskell

doi:10.1038/nmeth774

Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides

Robert J Beynon
, Mary K Doherty
, Julie M Pratt
, Simon J Gaskell

Research output: Contribution to journal › Article › peer-review

423 Citations (Scopus)

Abstract

Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.

Original language	English
Pages (from-to)	587-9
Number of pages	3
Journal	Nature Methods
Volume	2
Issue number	8
DOIs	https://doi.org/10.1038/nmeth774
Publication status	Published - Aug 2005

Keywords

Animals
Chickens
Gene Expression Profiling
Muscle Proteins
Peptide Mapping
Peptides
Proteome
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Staining and Labeling

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10.1038/nmeth774

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title = "Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides",

abstract = "Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.",

keywords = "Animals, Chickens, Gene Expression Profiling, Muscle Proteins, Peptide Mapping, Peptides, Proteome, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staining and Labeling",

author = "Beynon, \{Robert J\} and Doherty, \{Mary K\} and Pratt, \{Julie M\} and Gaskell, \{Simon J\}",

year = "2005",

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doi = "10.1038/nmeth774",

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journal = "Nature Methods",

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T1 - Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides

AU - Beynon, Robert J

AU - Doherty, Mary K

AU - Pratt, Julie M

AU - Gaskell, Simon J

PY - 2005/8

Y1 - 2005/8

N2 - Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.

AB - Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.

KW - Animals

KW - Chickens

KW - Gene Expression Profiling

KW - Muscle Proteins

KW - Peptide Mapping

KW - Peptides

KW - Proteome

KW - Proteomics

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Staining and Labeling

U2 - 10.1038/nmeth774

DO - 10.1038/nmeth774

M3 - Article

C2 - 16094383

SN - 1548-7091

VL - 2

SP - 587

EP - 589

JO - Nature Methods

JF - Nature Methods

IS - 8

ER -

Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides

Abstract

Keywords

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