Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides

Robert J Beynon, Mary K Doherty, Julie M Pratt, Simon J Gaskell

Research output: Contribution to journalArticle

352 Citations (Scopus)

Abstract

Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.
Original languageEnglish
Pages (from-to)587-9
Number of pages3
JournalNature Methods
Volume2
Issue number8
DOIs
Publication statusPublished - Aug 2005

    Fingerprint

Keywords

  • Animals
  • Chickens
  • Gene Expression Profiling
  • Muscle Proteins
  • Peptide Mapping
  • Peptides
  • Proteome
  • Proteomics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Staining and Labeling

Cite this