Molecular phylogeny of Pisolithus species from Moroccan forest woodlands

Salah Eddine Bakkali Yakhlef, Daniel Mousain, Robin Duponnois, Marc Ducousso, Abdellatif Belkouri, Benaissa Kerdouh, Marie-mathilde Perrineau, Mohamed Abourouh

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The phylogenetic relationships among 200 Pisolithus basidiomata collected from pine, oak, and eucalypt forests and rockrose scrubs in Morocco were investigated. Using PCR-RFLP analysis of the internal transcribed spacer (ITS) of ribosomal DNA, this collection could be divided into 5 groups, by using PCR-RFLP analysis of the internal transcribed spacer (ITS) of ribosomal DNA. The ITS of a representative basidioma of each group was sequenced, and a phylogenetic analysis was performed. The dendrogram suggests that at least five Pisolithus species are present in Morocco. Pisolithus basidiomata collected in the Pinus and Quercus forests correspond to Pisolithus arrhizus and P. species 4 as previously described by Martin and colleagues in 2002. Those collected from the eucalyptus forests, under E. gomphocephala and E. camaldulensis, correspond to P. albus and P. microcarpus. Basidiomata collected from the rockrose scrubs, under Cistus crispus, C. monspeliensis or C. salviifolius, are all identified as Pisolithus species 3. Phylogenetic analyses showed that our different Pisolithus grouped well with Pisolithus specimens from other geographical origins.
Original languageEnglish
Pages (from-to)157-162
Number of pages5
Issue number3
Publication statusPublished - 1 Dec 2009


  • Pisolithus
  • ectomycorrhizal fungus
  • phytogenetic analysis
  • ITS of ribosomal DNA
  • sequencing
  • taxonomy


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