TY - JOUR
T1 - Mitogen-activated protein kinase 14 promotes AKI
AU - Ortiz, Alberto
AU - Husi, Holger
AU - Gonzalez-Lafuente, Laura
AU - Valiño-Rivas, Lara
AU - Fresno, Manuel
AU - Sanz, Ana Belen
AU - Mullen, William
AU - Albalat, Amaya
AU - Mezzano, Sergio
AU - Vlahou, Tonia
AU - Mischak, Harald
AU - Sanchez-Niño, Maria Dolores
PY - 2017/3/1
Y1 - 2017/3/1
N2 - An improved understanding of pathogenic pathways in AKI may identify novel therapeutic approaches. Previously, we conducted unbiased liquid chromatography-tandem mass spectrometry-based protein expression profiling of the renal proteome in mice with acute folate nephropathy. Here, analysis of the dataset identified enrichment of pathways involving NFkappaB in the kidney cortex, and a targeted data mining approach identified components of the noncanonical NFkappaB pathway, including the upstream kinase mitogen-activated protein kinase kinase kinase 14 (MAP3K14), the NFkappaB DNA binding heterodimer RelB/NFkappaB2, and proteins involved in NFkappaB2 p100 ubiquitination and proteasomal processing to p52, as upregulated. Immunohistochemistry localized MAP3K14 expression to tubular cells in acute folate nephropathy and human AKI. In vivo, kidney expression levels of NFkappaB2 p100 and p52 increased rapidly after folic acid injection, as did DNA binding of RelB and NFkappaB2, detected in nuclei isolated from the kidneys. Compared with wild-type mice, MAP3K14 activity-deficient aly/aly (MAP3K14(aly/aly)) mice had less kidney dysfunction, inflammation, and apoptosis in acute folate nephropathy and less kidney dysfunction and a lower mortality rate in cisplatin-induced AKI. The exchange of bone marrow between wild-type and MAP3K14(aly/aly) mice did not affect the survival rate of either group after folic acid injection. In cultured tubular cells, MAP3K14 small interfering RNA targeting decreased inflammation and cell death. Additionally, cell culture and in vivo studies identified the chemokines MCP-1, RANTES, and CXCL10 as MAP3K14 targets in tubular cells. In conclusion, MAP3K14 promotes kidney injury through promotion of inflammation and cell death and is a promising novel therapeutic target.
AB - An improved understanding of pathogenic pathways in AKI may identify novel therapeutic approaches. Previously, we conducted unbiased liquid chromatography-tandem mass spectrometry-based protein expression profiling of the renal proteome in mice with acute folate nephropathy. Here, analysis of the dataset identified enrichment of pathways involving NFkappaB in the kidney cortex, and a targeted data mining approach identified components of the noncanonical NFkappaB pathway, including the upstream kinase mitogen-activated protein kinase kinase kinase 14 (MAP3K14), the NFkappaB DNA binding heterodimer RelB/NFkappaB2, and proteins involved in NFkappaB2 p100 ubiquitination and proteasomal processing to p52, as upregulated. Immunohistochemistry localized MAP3K14 expression to tubular cells in acute folate nephropathy and human AKI. In vivo, kidney expression levels of NFkappaB2 p100 and p52 increased rapidly after folic acid injection, as did DNA binding of RelB and NFkappaB2, detected in nuclei isolated from the kidneys. Compared with wild-type mice, MAP3K14 activity-deficient aly/aly (MAP3K14(aly/aly)) mice had less kidney dysfunction, inflammation, and apoptosis in acute folate nephropathy and less kidney dysfunction and a lower mortality rate in cisplatin-induced AKI. The exchange of bone marrow between wild-type and MAP3K14(aly/aly) mice did not affect the survival rate of either group after folic acid injection. In cultured tubular cells, MAP3K14 small interfering RNA targeting decreased inflammation and cell death. Additionally, cell culture and in vivo studies identified the chemokines MCP-1, RANTES, and CXCL10 as MAP3K14 targets in tubular cells. In conclusion, MAP3K14 promotes kidney injury through promotion of inflammation and cell death and is a promising novel therapeutic target.
KW - 3ref2021
U2 - 10.1681/ASN.2015080898
DO - 10.1681/ASN.2015080898
M3 - Article
SN - 1533-3450
VL - 28
SP - 823
EP - 836
JO - Journal of the American Society of Nephrology
JF - Journal of the American Society of Nephrology
IS - 3
ER -