A sensitive and reliable method is proposed allowing quantification of isoprostanes in salmon tissue using ultra-HPLC coupled to tandem MS. Solid phase extraction is used for sample concentration and purification. Repeatability, linearity, limits of detection and quantification were assessed by validation.
|Translated title of the contribution||METODA PRO ANALÝZU ISOPROSTAN Ů VE TKÁNI LOSOSA|
|Number of pages||7|
|Publication status||Published - Apr 2015|
- oxidative stress
- mass spectrometry
- liquid chromatography