Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids

Angelique Stalmach, Holger Husi, Khedidja Mosbahi, Amaya Albalat, William Mullen, Harald Mischak

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Proteomic biomarkers hold the promise of enabling assessment of patients according to a pathological condition aiming at improvements in diagnosis, prognosis, in general clinical patient management. Capillary electrophoresis coupled to an electrospray ionization time-of-flight mass spectrometer (CE-MS) allows the detection of thousands of small proteins and peptides in various biofluids, in a single, reproducible and time-limited step, enabling the simultaneous comparison of multiple individual proteins and peptides in biomarker discovery, but also in clinical applications. The reliability of the CE-MS platform, together with the use of a validated approach for data processing and mining is, to date, the most advanced technique for biomarker discovery of clinical significance. In this chapter, we report on the materials, methods and protocols used for CE-MS-based clinical proteomics allowing the reproducible profiling of biofluids.
Original languageEnglish
Pages (from-to)187-205
Number of pages19
JournalMethods in Molecular Biology
Volume1243
DOIs
Publication statusPublished - 2015

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