TY - JOUR
T1 - Methodology of fish eDNA and its applications in ecology and environment
AU - Wang, Shuping
AU - Yan, Zhenguang
AU - Hänfling, Bernd
AU - Zheng, Xin
AU - Wang, Pengyuan
AU - Fan, Juntao
AU - Li, Jianlong
N1 - Funding Information:
We wish to thank the members of the Yan Lab for helpful comments. Research in the Yan Lab is supported by the Fundamental Research Funds for Central Public Welfare Scientific Research Institutes of China [grant number 2019YSKY-007].
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2/10
Y1 - 2021/2/10
N2 - Fish environmental DNA (eDNA) studies have made substantial progress during the past decade, and significant advances in monitoring fishes have been gained by taking advantage of this technology. Although a number of reviews concerning eDNA are available and some recent fish eDNA reviews focused on fisheries or standard method have been published, a systematic review of methodology of fish eDNA and its applications in ecology and environment has not yet been published. To our knowledge, this is the first review of fish eDNA for solving ecological and environmental issues. First, the most comprehensive literature analysis of fish eDNA was presented and analyzed. Then, we systematically discuss the relevant experiments and analyses of fish eDNA, and infers that standard workflow is on the way to consensus. We additionally provide reference sequence databases and the primers used to amplify the reference sequences or detecting fish eDNA. The abiotic and biotic conditions affecting fish eDNA persistence are also summarized in a schematic diagram. Subsequently, we focus on the major achievements of fish eDNA in ecology and environment. We additionally highlight the exciting new tools, including in situ autonomous monitoring devices, CRISPR nucleic acid detection technology, and meta-omics technology for fish eDNA detection in future. Ultimately, methodology of fish eDNA will provide a wholly new paradigm for conservation actions of fishes, ecological and environmental management at a global scale.
AB - Fish environmental DNA (eDNA) studies have made substantial progress during the past decade, and significant advances in monitoring fishes have been gained by taking advantage of this technology. Although a number of reviews concerning eDNA are available and some recent fish eDNA reviews focused on fisheries or standard method have been published, a systematic review of methodology of fish eDNA and its applications in ecology and environment has not yet been published. To our knowledge, this is the first review of fish eDNA for solving ecological and environmental issues. First, the most comprehensive literature analysis of fish eDNA was presented and analyzed. Then, we systematically discuss the relevant experiments and analyses of fish eDNA, and infers that standard workflow is on the way to consensus. We additionally provide reference sequence databases and the primers used to amplify the reference sequences or detecting fish eDNA. The abiotic and biotic conditions affecting fish eDNA persistence are also summarized in a schematic diagram. Subsequently, we focus on the major achievements of fish eDNA in ecology and environment. We additionally highlight the exciting new tools, including in situ autonomous monitoring devices, CRISPR nucleic acid detection technology, and meta-omics technology for fish eDNA detection in future. Ultimately, methodology of fish eDNA will provide a wholly new paradigm for conservation actions of fishes, ecological and environmental management at a global scale.
KW - Ecology
KW - eDNA
KW - Environment
KW - Fish
KW - Methodology
UR - http://www.scopus.com/inward/record.url?scp=85092348615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85092348615&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2020.142622
DO - 10.1016/j.scitotenv.2020.142622
M3 - Review article
C2 - 33059148
AN - SCOPUS:85092348615
SN - 0048-9697
VL - 755
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 142622
ER -