Metabolomic analysis of Campylobacter jejuni by direct-injection electrospray ionization mass spectrometry

Robert M. Howlett, Matthew P. Davey, David J. Kelly

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the high-throughput and relatively simple sample preparation of DIMS has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. Here we describe a metabolomic fingerprinting approach with sampling and extraction methodologies optimized for direct-injection electrospray ionization mass spectrometry (ESI-MS), which we have used as a means of comparing wild-type and isogenic mutant strains of C. jejuni with various metabolic blocks.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages189-197
Number of pages9
ISBN (Electronic)978-1-4939-6536-6
ISBN (Print)978-1-4939-6534-2
DOIs
Publication statusPublished - 1 Jan 2017

Publication series

NameMethods in Molecular Biology
Volume1512
ISSN (Print)1064-3745

Keywords

  • DIMS
  • Direct injection
  • Electrospray ionization mass spectrometry
  • Fingerprinting
  • Metabolite extraction
  • Metabolome
  • Metabolomics

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