Metabolic recovery in herring larvae following strenuous activity

C E Franklin, I A Johnston, Robert S Batty, M C Yin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Larvae of spring spawning Clyde herring Clupea harengus L. were reared at 5 and 12 degrees C. Metabolism following burst swimming was studied in 7-day-old larvae at their respective rearing temperatures. Escape responses were repeatedly elicited using tactile stimulation for a period of 3 min. Larval herring were hard to Fatigue and still responded to tactile stimuli after 3 min. Whole larvae were freeze-quenched in liquid nitrogen, either immediately after exercise, or after periods of recovery of up to 24 h. Samples were freeze-dried and analysed for whole body creatine (Cr), phosphocreatine (PCr), ATP, ADP, AMP, lactate, glucose, and glycogen using high performance liquid chromatography and enzymatic methods. The exercise regime resulted in a marked decrease in PCr, ATP and glycogen concentrations and an increase in creatine, glucose and lactate concentrations whereas there was no significant change in either AMP or ADP concentrations. The extent of phosphagen hydrolysis (approx. 110 to 15 mu mol PCr g(-1) dry body mass) and lactate accumulation (approx. 7 to 40 mu mol lactate g(-1) dry body mass) over the exercise period was similar at the two temperatures, consistent with a relatively constant degree of effort. The rates of recovery of PCr and ATP were essentially the same at 5 and 12 degrees C; returning to resting levels after approximately 30 min. Lactate and glycogen concentrations were restored 60 min after exercise at both temperatures. Maximum lactate clearance rates (1.2 mu mol min(-1)g(-1) wet muscle mass) were an order of magnitude faster than reported for adult fish in the literature. (C) 1996 The Fisheries Society of the British isles
Original languageEnglish
Pages (from-to)207-216
Number of pages10
JournalJ FISH BIOL
Volume48
Issue number2
Publication statusPublished - 1996

Keywords

  • INTRACELLULAR ACID-BASE
  • CLUPEA-HARENGUS
  • PLEURONECTES-PLATESSA
  • RAINBOW-TROUT
  • Marine & Freshwater Biology
  • MUSCLE-FIBER TYPES
  • Fisheries
  • EXHAUSTIVE EXERCISE
  • SWIMMING MUSCLES
  • REARING TEMPERATURE
  • WHITE MUSCLE
  • FISH LARVAE

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