Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae)

N. Mesquita, C. Cunha, B. Hänfling, G. R. Carvalho, L. Zé-Zé, R. Tenreiro, M. M. Coelho

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Here we describe the isolation and characterization of six polymorphic microsatellite loci for Squalius aradensis. The number of observed alleles per locus ranged from three to 16 and the observed heterozygosity from 0.22 to 0.95. These primers will be useful in determining the population structure of S. aradensis and for conservation genetics of this endangered and endemic species. Furthermore, successful cross-species amplification in S. alburnoides and Chondrostoma lusitanicum suggests that a wider amplification of these markers is possible.

Original languageEnglish
Pages (from-to)572-574
Number of pages3
JournalMolecular Ecology Notes
Volume3
Issue number4
DOIs
Publication statusPublished - 9 Sept 2003

Keywords

  • Cyprinidae
  • di- and tetra-nucleotide repeats
  • Microsatellites
  • Primers
  • Squalius aradensis

Fingerprint

Dive into the research topics of 'Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae)'. Together they form a unique fingerprint.

Cite this