Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis

Ravindra Pal Singh; Mahendra K. Shukla; Avinash Mishra; Puja Kumari; C. R.K. Reddy; Bhavanath Jha

doi:10.1016/j.carbpol.2010.12.061

Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis

Ravindra Pal Singh, Mahendra K. Shukla, Avinash Mishra, Puja Kumari, C. R.K. Reddy, Bhavanath Jha

SAMS UHI

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170 Citations (Scopus)

Abstract

In the present study, EPS secreted by the endophytic bacterium Bacillus licheniformis was isolated and characterized. The molecular masses of the EPS were 1540 and 44,565 kDa and 1H NMR, FT-IR and UV-Vis spectral analyses revealed prevalence of characteristic primary amine-group, aromatic-compound, halide and aliphatic alkyl-group in addition to Na, P, Ca, C, O, Cl and S as inferred from EDX analysis. XRD and DSC analysis confirmed the amorphous nature of EPS, showing an average particle size of 24.977 μm (d 0.5) with 191 nm average roughness. The positive ion reflector mode of MALDI TOF-TOF MS exhibited a series of low and high mass peaks corresponding to various oligosaccharides and polysaccharides respectively. Further, GC-MS analysis revealed its four monosaccharide constituents glucose, galactose, mannose and arabinose. The potential heterogeneous properties of EPS as revealed in the present study may be explored in various biotechnological and industrial applications.

Original language	English
Pages (from-to)	1019-1026
Number of pages	8
Journal	Carbohydrate Polymers
Volume	84
Issue number	3
Early online date	25 Dec 2010
DOIs	https://doi.org/10.1016/j.carbpol.2010.12.061
Publication status	Published - 17 Mar 2011

Keywords

Atomic force microscopy
Bacillus licheniformis
Exopolysaccharide
MALDI-TOF-TOF MS
Seaweed
X-ray diffraction

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@article{9e6bbf4495194549b85abdd1f6a39900,

title = "Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis",

abstract = "In the present study, EPS secreted by the endophytic bacterium Bacillus licheniformis was isolated and characterized. The molecular masses of the EPS were 1540 and 44,565 kDa and 1H NMR, FT-IR and UV-Vis spectral analyses revealed prevalence of characteristic primary amine-group, aromatic-compound, halide and aliphatic alkyl-group in addition to Na, P, Ca, C, O, Cl and S as inferred from EDX analysis. XRD and DSC analysis confirmed the amorphous nature of EPS, showing an average particle size of 24.977 μm (d 0.5) with 191 nm average roughness. The positive ion reflector mode of MALDI TOF-TOF MS exhibited a series of low and high mass peaks corresponding to various oligosaccharides and polysaccharides respectively. Further, GC-MS analysis revealed its four monosaccharide constituents glucose, galactose, mannose and arabinose. The potential heterogeneous properties of EPS as revealed in the present study may be explored in various biotechnological and industrial applications.",

keywords = "Atomic force microscopy, Bacillus licheniformis, Exopolysaccharide, MALDI-TOF-TOF MS, Seaweed, X-ray diffraction",

author = "Singh, {Ravindra Pal} and Shukla, {Mahendra K.} and Avinash Mishra and Puja Kumari and Reddy, {C. R.K.} and Bhavanath Jha",

note = "Funding Information: The financial support received from the Council of Scientific and Industrial Research (NWP-018), New Delhi is gratefully acknowledged. The authors PK (CSIR-JRF), RPS (Project-SRF) and MKS (Project-JRF) gratefully acknowledge the CSIR, New Delhi for their fellowship. Copyright {\textcopyright} 2010 Elsevier Ltd. All rights reserved. The author was not affiliated to SAMS at the time of publication",

year = "2011",

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doi = "10.1016/j.carbpol.2010.12.061",

language = "English",

volume = "84",

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TY - JOUR

T1 - Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis

AU - Singh, Ravindra Pal

AU - Shukla, Mahendra K.

AU - Mishra, Avinash

AU - Kumari, Puja

AU - Reddy, C. R.K.

AU - Jha, Bhavanath

N1 - Funding Information: The financial support received from the Council of Scientific and Industrial Research (NWP-018), New Delhi is gratefully acknowledged. The authors PK (CSIR-JRF), RPS (Project-SRF) and MKS (Project-JRF) gratefully acknowledge the CSIR, New Delhi for their fellowship. Copyright © 2010 Elsevier Ltd. All rights reserved. The author was not affiliated to SAMS at the time of publication

PY - 2011/3/17

Y1 - 2011/3/17

N2 - In the present study, EPS secreted by the endophytic bacterium Bacillus licheniformis was isolated and characterized. The molecular masses of the EPS were 1540 and 44,565 kDa and 1H NMR, FT-IR and UV-Vis spectral analyses revealed prevalence of characteristic primary amine-group, aromatic-compound, halide and aliphatic alkyl-group in addition to Na, P, Ca, C, O, Cl and S as inferred from EDX analysis. XRD and DSC analysis confirmed the amorphous nature of EPS, showing an average particle size of 24.977 μm (d 0.5) with 191 nm average roughness. The positive ion reflector mode of MALDI TOF-TOF MS exhibited a series of low and high mass peaks corresponding to various oligosaccharides and polysaccharides respectively. Further, GC-MS analysis revealed its four monosaccharide constituents glucose, galactose, mannose and arabinose. The potential heterogeneous properties of EPS as revealed in the present study may be explored in various biotechnological and industrial applications.

AB - In the present study, EPS secreted by the endophytic bacterium Bacillus licheniformis was isolated and characterized. The molecular masses of the EPS were 1540 and 44,565 kDa and 1H NMR, FT-IR and UV-Vis spectral analyses revealed prevalence of characteristic primary amine-group, aromatic-compound, halide and aliphatic alkyl-group in addition to Na, P, Ca, C, O, Cl and S as inferred from EDX analysis. XRD and DSC analysis confirmed the amorphous nature of EPS, showing an average particle size of 24.977 μm (d 0.5) with 191 nm average roughness. The positive ion reflector mode of MALDI TOF-TOF MS exhibited a series of low and high mass peaks corresponding to various oligosaccharides and polysaccharides respectively. Further, GC-MS analysis revealed its four monosaccharide constituents glucose, galactose, mannose and arabinose. The potential heterogeneous properties of EPS as revealed in the present study may be explored in various biotechnological and industrial applications.

KW - Atomic force microscopy

KW - Bacillus licheniformis

KW - Exopolysaccharide

KW - MALDI-TOF-TOF MS

KW - Seaweed

KW - X-ray diffraction

U2 - 10.1016/j.carbpol.2010.12.061

DO - 10.1016/j.carbpol.2010.12.061

M3 - Article

SN - 0144-8617

VL - 84

SP - 1019

EP - 1026

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 3

ER -

Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis

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