Introducing the Concept and Application of Vitrification for the Cryo-conservation of Algae "A Mini Review".

Keith Harding, John Day, Maike Lorenz, H Timmerman, Thomas Friedl, David H Bremner, Erica E Benson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This review pertains to a project (COBRA QLRT-2000-01645, http://www.cobra.ac.uk) supported by the European Commission's Fifth Framework Programme for Quality of Life and Management of Living Resources. The aim of this initiative is to develop and apply novel cryo-preservation protocols to microalgae and cyanobacteria that are difficult to cryopreserve. One such approach utilizes cryoprotective vitrification for the conservation of algal culture collections at -196degreesC in liquid nitrogen. Achieving a vitrified state depends on increasing cellular viscosity to a critical point at which water forms an amorphous, non-crystalline glass on cooling to ultra-low temperatures. Vitrification has been utilized extensively in medical cryobiology and for the preservation of animal, human and higher plant germplasm but its application for the conservation of algae and cyarlobacteria is less widespread. The review provides a theoretical introduction to vitrification and highlights current and potential applications in algal preservation, especially for storage-recalcitrant organisms.
Original languageEnglish
Pages (from-to)207-226
Number of pages20
JournalNOVA HEDWIGIA
Issue number9
DOIs
Publication statusPublished - 2004

Keywords

  • CRYOINJURY
  • DESICCATION
  • SOMATIC EMBRYOS
  • VAUCHERIA-SESSILIS
  • EUGLENA-GRACILIS
  • SHOOT-TIPS
  • CRYOPRESERVATION-RECALCITRANCE
  • EUKARYOTIC ALGAE
  • ENCAPSULATION-DEHYDRATION
  • MICROALGAE

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    Harding, K., Day, J., Lorenz, M., Timmerman, H., Friedl, T., Bremner, D. H., & Benson, E. E. (2004). Introducing the Concept and Application of Vitrification for the Cryo-conservation of Algae "A Mini Review". NOVA HEDWIGIA, (9), 207-226. https://doi.org/10.1127/0029-5035/2004/0079-0207