Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

R. G. Barlow; D. G. Cummings; S. W. Gibb

doi:10.3354/meps161303

Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

R. G. Barlow
, D. G. Cummings
, S. W. Gibb

Environmental Research Institute

Research output: Contribution to journal › Article › peer-review

273 Citations (Scopus)

Abstract

A reverse phase HPLC (high performance liquid chromatography) technique using a binary solvent system with a linear gradient on a Hypersil® MOS2 C-8 column is described. As well as the resolution of key chemotaxonomic chlorophyll and carotenoid pigments, baseline separation of mono- and divinyl chlorophyll a and of lutein and zeaxanthin, and partial separation of mono- and divinyl chlorophyll b are achieved in a total analysis time of less than 30 min. The method provides an optimal balance between analyte resolution and sample throughput and is hence well suited to the analysis of oceanographic samples.

Original language	English
Pages (from-to)	303-307
Number of pages	5
Journal	Marine Ecology Progress Series
Volume	161
DOIs	https://doi.org/10.3354/meps161303
Publication status	Published - 31 Dec 1997

Keywords

C-8 column
Carotenoids
Chlorophylls
HPLC
Improved resolution

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10.3354/meps161303

Cite this

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title = "Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC",

abstract = "A reverse phase HPLC (high performance liquid chromatography) technique using a binary solvent system with a linear gradient on a Hypersil{\textregistered} MOS2 C-8 column is described. As well as the resolution of key chemotaxonomic chlorophyll and carotenoid pigments, baseline separation of mono- and divinyl chlorophyll a and of lutein and zeaxanthin, and partial separation of mono- and divinyl chlorophyll b are achieved in a total analysis time of less than 30 min. The method provides an optimal balance between analyte resolution and sample throughput and is hence well suited to the analysis of oceanographic samples.",

keywords = "C-8 column, Carotenoids, Chlorophylls, HPLC, Improved resolution",

author = "Barlow, \{R. G.\} and Cummings, \{D. G.\} and Gibb, \{S. W.\}",

year = "1997",

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language = "English",

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T1 - Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

AU - Barlow, R. G.

AU - Cummings, D. G.

AU - Gibb, S. W.

PY - 1997/12/31

Y1 - 1997/12/31

N2 - A reverse phase HPLC (high performance liquid chromatography) technique using a binary solvent system with a linear gradient on a Hypersil® MOS2 C-8 column is described. As well as the resolution of key chemotaxonomic chlorophyll and carotenoid pigments, baseline separation of mono- and divinyl chlorophyll a and of lutein and zeaxanthin, and partial separation of mono- and divinyl chlorophyll b are achieved in a total analysis time of less than 30 min. The method provides an optimal balance between analyte resolution and sample throughput and is hence well suited to the analysis of oceanographic samples.

AB - A reverse phase HPLC (high performance liquid chromatography) technique using a binary solvent system with a linear gradient on a Hypersil® MOS2 C-8 column is described. As well as the resolution of key chemotaxonomic chlorophyll and carotenoid pigments, baseline separation of mono- and divinyl chlorophyll a and of lutein and zeaxanthin, and partial separation of mono- and divinyl chlorophyll b are achieved in a total analysis time of less than 30 min. The method provides an optimal balance between analyte resolution and sample throughput and is hence well suited to the analysis of oceanographic samples.

KW - C-8 column

KW - Carotenoids

KW - Chlorophylls

KW - HPLC

KW - Improved resolution

UR - https://www.scopus.com/pages/publications/0031593009

UR - https://www.scopus.com/pages/publications/0031593009#tab=citedBy

U2 - 10.3354/meps161303

DO - 10.3354/meps161303

M3 - Article

AN - SCOPUS:0031593009

SN - 0171-8630

VL - 161

SP - 303

EP - 307

JO - Marine Ecology Progress Series

JF - Marine Ecology Progress Series

ER -

Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

Abstract

Keywords

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