HoxB8 neutrophils replicate Fcγ receptor and integrin‐induced neutrophil signaling and functions

Julia Y. Chu, Barry Mccormick, Greta Mazelyte, Melina Michael, Sonja Vermeren

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Neutrophils are short‐lived, terminally differentiated leukocytes that form an essential part of host immunity and play a key role in acute and chronic inflammation. The analysis of these important cells is hindered by the fact that neutrophils are not amenable to culture, transfection, or transduction. Conditionally HoxB8‐immortalized mouse hematopoietic progenitors are suitable for in vitro differentiation of a range of myeloid cells, including neutrophils. Integrins and FcγRs are cell surface receptors, the ligation of which is required for a range of neutrophil functions that are important in health and disease. We show here that HoxB8 neutrophils express major neutrophil integrins and FcγRs. They respond to FcγR and integrin stimulation in a manner that is comparable with primary neutrophils, in terms of intracellular signaling. HoxB8 neutrophils also perform a range of FcγR/integrin‐dependent neutrophil functions, including, generation of reactive oxygen species, degranulation, and chemotaxis. Our findings suggest that HoxB8 neutrophils represent a faithful experimental model system for the analysis of Fc and integrin receptor‐dependent neutrophil functions.
Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalJournal of Leukocyte Biology
Volume105
Issue number1
Early online date27 Dec 2018
DOIs
Publication statusPublished - 31 Jan 2019

Keywords

  • granulocytes
  • human cell lines
  • monocytes/macrophages
  • neutrophils
  • adhesion molecules
  • intra-cellular signaling
  • Fc Receptors
  • transgenic/knockout mice

Fingerprint Dive into the research topics of 'HoxB8 neutrophils replicate Fcγ receptor and integrin‐induced neutrophil signaling and functions'. Together they form a unique fingerprint.

  • Cite this