Evidence of a latent oxidative burst in relation to wound repair in the giant unicellular chlorophyte Dasycladus vermicularis

Clifford Ross, Frithjof Küpper, Valerie Vreeland, J Herbert Waite, Robert S Jacobs

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We investigated the kinetics and composition of the second phase of the wound repair process of Dasycladus vermicularis ([Scropoli] Krasser) using fluorescent probes, chromatography, UV spectroscopy, and histochemistry. Our new evidence supports the hypothesis that the second phase of wound repair (initiated at approximately 35-45 min postinjury) is based on the activation of an oxidative burst that produces micromolar H2O2 levels. These results provide evidence of peroxidase activity at the wound site, real-time measurements of an oxidative burst, and catechol localization in wound plugs. Strong evidence is presented indicating that the biochemical machinery exists for oxidative cross-linking to ensue in the wound-healing process of D. vermicularis.
Original languageEnglish
Pages (from-to)531-541
Number of pages11
JournalJ PHYCOL
Volume41
Issue number3
DOIs
Publication statusPublished - 2005

Keywords

  • OXYGEN PRODUCTION
  • PLANT-DISEASE RESISTANCE
  • ALGAE CLADOPHORA-GLOMERATA
  • CROSS-LINKING
  • Marine & Freshwater Biology
  • CELLS
  • FERULIC ACID
  • HYDROGEN-PEROXIDE
  • NEUTROPHIL NADPH OXIDASE
  • MECHANISM
  • Plant Sciences
  • WALL STRUCTURAL PROTEINS

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