Development of a pyrroloquinoline quinone (PQQ) mediated glucose oxidase enzyme electrode for detection of glucose in fruit juice

Michael G. Loughran; Jenny M. Hall; Anthony P. F. Turner

doi:10.1002/elan.1140081004

Development of a pyrroloquinoline quinone (PQQ) mediated glucose oxidase enzyme electrode for detection of glucose in fruit juice

Michael G. Loughran, Jenny M. Hall, Anthony P. F. Turner

Division of Rural Health and Wellbeing

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

The electrochemistry of pyrroloquinoline quinone (PQQ) was examined and the coupling of PQQ with glucose oxidase was studied at different pH values. Optimal interaction between PQQ and glucose oxidase was observed at pH 3.5. Results from these studies enabled construction of PQQ-glucose oxidase-modified enzyme electrodes for the detection of glucose in Ribena blackcurrant juice. The corresponding amperometric determination of glucose was in good agreement with results obtained using commercial enzymatic test kits supplied by Bochringer Mannheim and Sigma Diagnostics.

Original language	English
Pages (from-to)	870-875
Number of pages	5
Journal	Electroanalysis
Volume	8
Issue number	10
DOIs	https://doi.org/10.1002/elan.1140081004
Publication status	Published - 1 Oct 1996

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10.1002/elan.1140081004

http://doi.wiley.com/10.1002/elan.1140081004

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title = "Development of a pyrroloquinoline quinone (PQQ) mediated glucose oxidase enzyme electrode for detection of glucose in fruit juice",

abstract = "The electrochemistry of pyrroloquinoline quinone (PQQ) was examined and the coupling of PQQ with glucose oxidase was studied at different pH values. Optimal interaction between PQQ and glucose oxidase was observed at pH 3.5. Results from these studies enabled construction of PQQ-glucose oxidase-modified enzyme electrodes for the detection of glucose in Ribena blackcurrant juice. The corresponding amperometric determination of glucose was in good agreement with results obtained using commercial enzymatic test kits supplied by Bochringer Mannheim and Sigma Diagnostics.",

author = "Loughran, {Michael G.} and Hall, {Jenny M.} and Turner, {Anthony P. F.}",

year = "1996",

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TY - JOUR

T1 - Development of a pyrroloquinoline quinone (PQQ) mediated glucose oxidase enzyme electrode for detection of glucose in fruit juice

AU - Loughran, Michael G.

AU - Hall, Jenny M.

AU - Turner, Anthony P. F.

PY - 1996/10/1

Y1 - 1996/10/1

N2 - The electrochemistry of pyrroloquinoline quinone (PQQ) was examined and the coupling of PQQ with glucose oxidase was studied at different pH values. Optimal interaction between PQQ and glucose oxidase was observed at pH 3.5. Results from these studies enabled construction of PQQ-glucose oxidase-modified enzyme electrodes for the detection of glucose in Ribena blackcurrant juice. The corresponding amperometric determination of glucose was in good agreement with results obtained using commercial enzymatic test kits supplied by Bochringer Mannheim and Sigma Diagnostics.

AB - The electrochemistry of pyrroloquinoline quinone (PQQ) was examined and the coupling of PQQ with glucose oxidase was studied at different pH values. Optimal interaction between PQQ and glucose oxidase was observed at pH 3.5. Results from these studies enabled construction of PQQ-glucose oxidase-modified enzyme electrodes for the detection of glucose in Ribena blackcurrant juice. The corresponding amperometric determination of glucose was in good agreement with results obtained using commercial enzymatic test kits supplied by Bochringer Mannheim and Sigma Diagnostics.

U2 - 10.1002/elan.1140081004

DO - 10.1002/elan.1140081004

M3 - Article

SN - 1040-0397

VL - 8

SP - 870

EP - 875

JO - Electroanalysis

JF - Electroanalysis

IS - 10

ER -

Development of a pyrroloquinoline quinone (PQQ) mediated glucose oxidase enzyme electrode for detection of glucose in fruit juice

Abstract

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