TY - JOUR
T1 - Desiccation induced oxidative stress and its biochemical responses in intertidal red alga Gracilaria corticata (Gracilariales, Rhodophyta)
AU - Kumar, Manoj
AU - Gupta, Vishal
AU - Trivedi, Nitin
AU - Kumari, Puja
AU - Bijo, A. J.
AU - Reddy, C. R.K.
AU - Jha, Bhavanath
N1 - Funding Information: The financial support received from CSIR (NWP-018) is gratefully acknowledged. The first (MK) and fourth author (PK) gratefully acknowledge the CSIR, New Delhi, for awarding the Senior and Junior Research Fellowships respectively. The second (VG), third (NT) and fifth (AJB) author also express their gratitude to CSIR (RSP-016) for financial support.
Copyright © 2011 Elsevier B.V. All rights reserved.
The author was not affiliated to SAMS at the time of publication
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Intertidal alga Gracilaria corticata growing in natural environment experiences various abiotic stresses during the low tides. The aim of this study was to determine whether desiccation exposure would lead to oxidative stress and its effect varies with exposure periods. This study gives an account of various biochemical changes in G. corticata following the exposure to desiccation for a period of 0 (control), 1, 2, 3 and 4. h under controlled conditions. During desiccation, G. corticata thalli showed dramatic loss of water by almost 47% when desiccated for 4. h. The enhanced production of reactive oxygen species (ROS) and increased lipid peroxidation observed during the exposure of 3-4. h were chiefly contributed by higher lipoxygenase (LOX) activity with the induction of two new LOX isoforms (LOX-2, ~85. kDa; LOX-3, ~65. kDa). The chlorophyll, carotenoids and phycobiliproteins (phycoerythrin and phycocyanin) were increased during initial 2. h exposure compared to control and thereafter declined in the succeeding exposure. The antioxidative enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione peroxidase (GPX) and the regeneration rate of reduced ascorbate (AsA) and glutathione (GSH) increased during desiccation up to 2-3. h. Further, the isoforms of antioxidant enzymes Mn-SOD (~150. kDa), APX-4 (~110. kDa), APX-5 (~45. kDa), GPX-1 (~80. kDa) and GPX-2 (~65. kDa) responded specifically to the desiccation exposure. Compared to control, a relative higher content of both free and bound insoluble putrescine and spermine together with enhanced n-6 PUFAs namely C20:4(n-6) and C20:3(n-6) fatty acids found during 2. h exposure reveals their involvement in defence reactions against the desiccation induced oxidative stress.
AB - Intertidal alga Gracilaria corticata growing in natural environment experiences various abiotic stresses during the low tides. The aim of this study was to determine whether desiccation exposure would lead to oxidative stress and its effect varies with exposure periods. This study gives an account of various biochemical changes in G. corticata following the exposure to desiccation for a period of 0 (control), 1, 2, 3 and 4. h under controlled conditions. During desiccation, G. corticata thalli showed dramatic loss of water by almost 47% when desiccated for 4. h. The enhanced production of reactive oxygen species (ROS) and increased lipid peroxidation observed during the exposure of 3-4. h were chiefly contributed by higher lipoxygenase (LOX) activity with the induction of two new LOX isoforms (LOX-2, ~85. kDa; LOX-3, ~65. kDa). The chlorophyll, carotenoids and phycobiliproteins (phycoerythrin and phycocyanin) were increased during initial 2. h exposure compared to control and thereafter declined in the succeeding exposure. The antioxidative enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione peroxidase (GPX) and the regeneration rate of reduced ascorbate (AsA) and glutathione (GSH) increased during desiccation up to 2-3. h. Further, the isoforms of antioxidant enzymes Mn-SOD (~150. kDa), APX-4 (~110. kDa), APX-5 (~45. kDa), GPX-1 (~80. kDa) and GPX-2 (~65. kDa) responded specifically to the desiccation exposure. Compared to control, a relative higher content of both free and bound insoluble putrescine and spermine together with enhanced n-6 PUFAs namely C20:4(n-6) and C20:3(n-6) fatty acids found during 2. h exposure reveals their involvement in defence reactions against the desiccation induced oxidative stress.
KW - Antioxidative enzymes
KW - Desiccation
KW - Gracilaria corticata
KW - Polyamines
KW - PUFAs
KW - Reactive oxygen species
U2 - 10.1016/j.envexpbot.2011.03.007
DO - 10.1016/j.envexpbot.2011.03.007
M3 - Article
SN - 0098-8472
VL - 72
SP - 194
EP - 201
JO - Environmental and Experimental Botany
JF - Environmental and Experimental Botany
IS - 2
ER -