Complementation cloning of salt tolerance determinants from the marine hyphomycete Dendryphiella salina in Aspergillus nidulans

D. J. Clement, M. S. Stanley, J. O'Neil, N. A. Woodcock, D. A. Fincham, N. J.W. Clipson, P. Hooley

Research output: Contribution to journalArticlepeer-review

Abstract

A preliminary physiological characterization of osmoregulation in an Aspergillus nidulans salt sensitive mutant carrying cotransformed DNA sequences from the marine hyphomycete Dendryphiella salina is described. A genomic DNA library made in lambda EMBL3 was cotransformed with the plasmid pDJB3 into an A. nidulans host carrying a selectable marker for transformation (pyrG89) and a salt sensitivity mutation (sltA1). In some cases D. salina clones appeared to directly complement the salt sensitivity mutation by restoring salt and arginine insensitivity. Other clones gave marked increases in salt tolerance and yet the hosts remained arginine sensitive. Improvements in salt tolerance correlated with an enhanced ability to induce polyol biosynthesis during salt challenge.

Original languageEnglish
Pages (from-to)1252-1258
Number of pages7
JournalMycological Research
Volume103
Issue number10
DOIs
Publication statusPublished - 1 Oct 1999

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