TY - JOUR
T1 - Chytrid infecting the bloom-forming marine diatom Skeletonema sp.:
T2 - Morphology, phylogeny and distribution of a novel species within the Rhizophydiales
AU - Garvetto, Andrea
AU - Badis, Yacine
AU - Perrineau, Marie-mathilde
AU - Rad-menéndez, Cecilia
AU - Bresnan, Eileen
AU - Gachon, Claire M.m.
N1 - © 2019 Published by Elsevier Ltd on behalf of British Mycological Society.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Chytrids have long been recognised as important parasites of microalgae in freshwater systems, able to shape the dynamics of blooms, the gene pool of their host and phytoplankton succession. In the sea however, where the presence of these organisms is erratic and ephemeral, studies concerning chytrids are sparse and confined to metabarcoding surveys or microscopy observations. Despite the scarcity of data, chytrid epidemics are supposed to play an important role in marine biogeochemical cycles, being one of the drivers of phytoplankton dynamics. Here we combine microscopy observations and in silico mining of a single-cell whole genome to molecularly and morphologically characterise a novel chytrid parasite of the dominant diatom genus Skeletonema. Morphological observations highlight features of the thallus and ascertain the parasitic nature of the interaction whilst the genetic markers obtained allows for a phylogenetic reconstruction, placing the new species in the order Rhizophydiales. Thanks to the molecular data obtained we are also able to provide a first investigation of the global distribution of this organism by screening the Ocean Sampling Day (OSD) dataset, highlighting a northern transatlantic dissemination.
AB - Chytrids have long been recognised as important parasites of microalgae in freshwater systems, able to shape the dynamics of blooms, the gene pool of their host and phytoplankton succession. In the sea however, where the presence of these organisms is erratic and ephemeral, studies concerning chytrids are sparse and confined to metabarcoding surveys or microscopy observations. Despite the scarcity of data, chytrid epidemics are supposed to play an important role in marine biogeochemical cycles, being one of the drivers of phytoplankton dynamics. Here we combine microscopy observations and in silico mining of a single-cell whole genome to molecularly and morphologically characterise a novel chytrid parasite of the dominant diatom genus Skeletonema. Morphological observations highlight features of the thallus and ascertain the parasitic nature of the interaction whilst the genetic markers obtained allows for a phylogenetic reconstruction, placing the new species in the order Rhizophydiales. Thanks to the molecular data obtained we are also able to provide a first investigation of the global distribution of this organism by screening the Ocean Sampling Day (OSD) dataset, highlighting a northern transatlantic dissemination.
KW - Marine fungi
KW - marine microbial diversity
KW - NGS
KW - Plankton parasites
KW - Single-cell analysis
U2 - 10.1016/j.funbio.2019.04.004
DO - 10.1016/j.funbio.2019.04.004
M3 - Article
SN - 1878-6146
VL - 123
SP - 471
EP - 480
JO - Fungal Biology
JF - Fungal Biology
IS - 6
ER -