CARBODIIMIDE-INDUCED LABELING OF SEA-URCHIN SURFACES (ECHINOIDEA, ECHINODERMATA)

I V Grigolava; J D McKenzie

CARBODIIMIDE-INDUCED LABELING OF SEA-URCHIN SURFACES (ECHINOIDEA, ECHINODERMATA)

I V Grigolava, J D McKenzie

SAMS UHI

Research output: Contribution to journal › Article › peer-review

Abstract

A method for radiolabelling echinoderm body surfaces, using water-soluble carbodiimide was developed. The number of carboxyl groups reacting with carbodiimide was estimated as 20 to 30 nmole g(-1) dry weight of sea urchin spines. There were no phosphate groups accessible to carbodiimide on the surface coat. Several enzymes were used to release bound radiolabel from the spines labelled with carbodiimide and radioactive amino acid, Results obtained with proteases and hyaluronidase showed that radiolabel was bound to the glycoprotein and/or protein molecules on the extracellular surface of the spine epidermis.

Original language	English
Pages (from-to)	477-482
Number of pages	6
Journal	COMP BIOCHEM PHYS B
Volume	110
Issue number	3
Publication status	Published - 1995

Keywords

Biochemistry & Molecular Biology
Zoology
ULTRASTRUCTURE

Handle.net

Persistent link

Cite this

@article{7e29cf7cbd324bc4bf360ccc84b27793,

title = "CARBODIIMIDE-INDUCED LABELING OF SEA-URCHIN SURFACES (ECHINOIDEA, ECHINODERMATA)",

abstract = "A method for radiolabelling echinoderm body surfaces, using water-soluble carbodiimide was developed. The number of carboxyl groups reacting with carbodiimide was estimated as 20 to 30 nmole g(-1) dry weight of sea urchin spines. There were no phosphate groups accessible to carbodiimide on the surface coat. Several enzymes were used to release bound radiolabel from the spines labelled with carbodiimide and radioactive amino acid, Results obtained with proteases and hyaluronidase showed that radiolabel was bound to the glycoprotein and/or protein molecules on the extracellular surface of the spine epidermis.",

keywords = "Biochemistry & Molecular Biology, Zoology, ULTRASTRUCTURE",

author = "Grigolava, {I V} and McKenzie, {J D}",

year = "1995",

language = "English",

volume = "110",

pages = "477--482",

journal = "COMP BIOCHEM PHYS B",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - CARBODIIMIDE-INDUCED LABELING OF SEA-URCHIN SURFACES (ECHINOIDEA, ECHINODERMATA)

AU - Grigolava, I V

AU - McKenzie, J D

PY - 1995

Y1 - 1995

N2 - A method for radiolabelling echinoderm body surfaces, using water-soluble carbodiimide was developed. The number of carboxyl groups reacting with carbodiimide was estimated as 20 to 30 nmole g(-1) dry weight of sea urchin spines. There were no phosphate groups accessible to carbodiimide on the surface coat. Several enzymes were used to release bound radiolabel from the spines labelled with carbodiimide and radioactive amino acid, Results obtained with proteases and hyaluronidase showed that radiolabel was bound to the glycoprotein and/or protein molecules on the extracellular surface of the spine epidermis.

AB - A method for radiolabelling echinoderm body surfaces, using water-soluble carbodiimide was developed. The number of carboxyl groups reacting with carbodiimide was estimated as 20 to 30 nmole g(-1) dry weight of sea urchin spines. There were no phosphate groups accessible to carbodiimide on the surface coat. Several enzymes were used to release bound radiolabel from the spines labelled with carbodiimide and radioactive amino acid, Results obtained with proteases and hyaluronidase showed that radiolabel was bound to the glycoprotein and/or protein molecules on the extracellular surface of the spine epidermis.

KW - Biochemistry & Molecular Biology

KW - Zoology

KW - ULTRASTRUCTURE

M3 - Article

VL - 110

SP - 477

EP - 482

JO - COMP BIOCHEM PHYS B

JF - COMP BIOCHEM PHYS B

IS - 3

ER -

CARBODIIMIDE-INDUCED LABELING OF SEA-URCHIN SURFACES (ECHINOIDEA, ECHINODERMATA)

Abstract

Keywords

Handle.net

Fingerprint

Cite this