Bradyrhizobia nodulating the Acacia mangium x A. auriculiformis interspecific hybrid are specific and differ from those associated with both parental species

Christine Le Roux, Diana Tentchev, Yves Prin, Doreen Goh, Yani Japarudin, Marie-Mathilde Perrineau, Robin Duponnois, Odile Domergue, Philippe de Lajudie, Antoine Galiana

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In the context of an increasing utilization of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree in the tropical humid zone, its symbiotic characterization was carried out in comparison with that of its two parental species. Rhizobium strains of diverse geographical origins were isolated from root nodules of the hybrid and its parents. Almost all Acacia hybrid isolates were fast growing on yeast extract-mannitol medium, in contrast to those isolated from both parental species, which were mostly slow growing. The rhizobium strains were characterized through partial sequencing of the rRNA operon. In the phylogenetic tree, almost all strains isolated from the hybrid were grouped together in a clade close to Bradyrhizobium japonicum, while all strains isolated from both parental species were close to Bradyrhizobium elkanii. Inoculation experiments performed under in vitro or greenhouse conditions showed that all strains were infective with their original hosts but exhibited very variable degrees of effectivity according to the host plant tested. Thus, homologous strain-host associations were more effective than heterologous ones. This shows that there is still a high potential for isolating and testing new strains from hybrids to be used as inoculants in the context of large-scale afforestation programs.

Original languageEnglish
Pages (from-to)7752-9
Number of pages8
JournalApplied and Environmental Microbiology
Volume75
Issue number24
DOIs
Publication statusPublished - Dec 2009

Keywords

  • Acacia
  • Animals
  • Biodiversity
  • Bradyrhizobium
  • Chimera
  • DNA, Bacterial
  • Molecular Sequence Data
  • Nitrogen Fixation
  • Phylogeny
  • RNA, Bacterial
  • RNA, Ribosomal
  • Root Nodules, Plant
  • Soil Microbiology
  • Species Specificity
  • Symbiosis
  • Journal Article

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