Association between protozoa in sputum and asthma: A case-control study

Hugo C. Van Woerden, Adriana Ratier-Cruz, Olabode B. Aleshinloye, Rafael Martinez-Giron, Clive Gregory, Ian P. Matthews

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9 Citations (Scopus)

Abstract

Background: Atypical infectious agents have been proposed as potential contributors to asthma. A novel set of morphological and staining criteria permit the identification of flagellated protozoa in sputum. This case-control study was designed to use this novel method and to assess: (1) are protozoa more common in asthmatics than in non-asthmatics; (2) is the presence of protozoa associated with the use of steroid inhalers; and (3) is the presence of protozoa associated with living in damp housing? Methods: Induced sputum samples were collected from asthma patients and local non-atopic, non-smoking controls. Questionnaires assessed asthma severity and housing conditions. Sputum was examined for flagellated protozoa using a previously described staining technique. Results: 96 participants were recruited for this study; 54 asthma patients and 42 controls, age range 21-62 years, 70% female participants. Limiting results to those who were clearly positive or negative for flagellated protozoa, 66.7% (20/30) of asthmatics and 30.8% (4/13) of controls had protozoa (p = 0.046). Among the asthma patients, prevalence of protozoa was not significantly different between those who had (10/18), and those who had not (10/12), used steroid inhaler in the preceding two weeks (p = 0.11). Similarly, the prevalence of protozoa was not significantly different between those who did (6/11) and those who did not (18/32), live in damp homes (p = 0.92). Conclusions: This case-control study demonstrates an association between flagellated protozoa in sputum and asthma. It is now necessary to confirm and characterise the protozoa using genetic techniques based on 18S ribosomal RNA. Once tis is established it would be worthwhile to determine if asthma symptoms improve when treated by anti-protozoal agents.

Original languageEnglish
Pages (from-to)877-884
Number of pages8
JournalRespiratory Medicine
Volume105
Issue number6
Early online date8 Dec 2010
DOIs
Publication statusPublished - 1 Jun 2011

Keywords

  • Asthma
  • Case-control study
  • Protozoa
  • Respiratory tract infections

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    Van Woerden, H. C., Ratier-Cruz, A., Aleshinloye, O. B., Martinez-Giron, R., Gregory, C., & Matthews, I. P. (2011). Association between protozoa in sputum and asthma: A case-control study. Respiratory Medicine, 105(6), 877-884. https://doi.org/10.1016/j.rmed.2010.11.016