Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

H C Reinardy, E Skippins, T B Henry, A N Jha

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

Original languageEnglish
Pages (from-to)1074-81
Number of pages8
JournalJournal of Fish Biology
Volume82
Issue number3
DOIs
Publication statusPublished - 7 Mar 2013

Fingerprint

Danio rerio
DNA damage
sperm
spermatozoa
DNA
damage
sampling
tail
comet
hydrogen peroxide
gel electrophoresis
electrokinesis
gel
assay
assays
fish

Keywords

  • Animals
  • DNA Damage
  • Hydrogen Peroxide/adverse effects
  • Male
  • Sperm Count
  • Spermatozoa/pathology
  • Zebrafish

Cite this

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title = "Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio",
abstract = "Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9{\%} tail DNA compared to unexposed controls [12 ± 0·7{\%} tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22{\%} for fish sampled every 2 or 4 days, respectively.",
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Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio. / Reinardy, H C; Skippins, E; Henry, T B; Jha, A N.

In: Journal of Fish Biology, Vol. 82, No. 3, 07.03.2013, p. 1074-81.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

AU - Reinardy, H C

AU - Skippins, E

AU - Henry, T B

AU - Jha, A N

N1 - © 2013 The Authors. Journal of Fish Biology © 2013 The Fisheries Society of the British Isles

PY - 2013/3/7

Y1 - 2013/3/7

N2 - Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

AB - Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

KW - Animals

KW - DNA Damage

KW - Hydrogen Peroxide/adverse effects

KW - Male

KW - Sperm Count

KW - Spermatozoa/pathology

KW - Zebrafish

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