Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

H C Reinardy, E Skippins, T B Henry, A N Jha

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Abstract

Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

Original languageEnglish
Pages (from-to)1074-81
Number of pages8
JournalJournal of Fish Biology
Volume82
Issue number3
DOIs
Publication statusPublished - 7 Mar 2013

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Keywords

  • Animals
  • DNA Damage
  • Hydrogen Peroxide/adverse effects
  • Male
  • Sperm Count
  • Spermatozoa/pathology
  • Zebrafish

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