Abstract
Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.
Original language | English |
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Pages (from-to) | 1074-81 |
Number of pages | 8 |
Journal | Journal of Fish Biology |
Volume | 82 |
Issue number | 3 |
DOIs | |
Publication status | Published - 7 Mar 2013 |
Keywords
- Animals
- DNA Damage
- Hydrogen Peroxide/adverse effects
- Male
- Sperm Count
- Spermatozoa/pathology
- Zebrafish