An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

Nitin Trivedi; Vishal Gupta; Manoj Kumar; Puja Kumari; C. R.K. Reddy; B. Jha

doi:10.1016/j.carbpol.2010.08.069

An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

Nitin Trivedi
, Vishal Gupta
, Manoj Kumar
, Puja Kumari
, C. R.K. Reddy
, B. Jha

SAMS UHI

Research output: Contribution to journal › Article › peer-review

113 Citations (Scopus)

Abstract

An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0-12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.

Original language	English
Pages (from-to)	891-897
Number of pages	7
Journal	Carbohydrate Polymers
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/j.carbpol.2010.08.069
Publication status	Published - 10 Jan 2011

Keywords

Cellulase
Cellulose
Halo-alkali tolerance
Marine habitat

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@article{b75749e5297242cca43f6ae73ef66108,

title = "An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca",

abstract = "An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03\% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0-12.0 and residual activity of about 70\% at salt concentration (NaCl 15\%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.",

keywords = "Cellulase, Cellulose, Halo-alkali tolerance, Marine habitat",

author = "Nitin Trivedi and Vishal Gupta and Manoj Kumar and Puja Kumari and Reddy, \{C. R.K.\} and B. Jha",

note = "Funding Information: The financial support received from CSIR (RSP 0016), New Delhi is gratefully acknowledged. We would also like to thank Dr. P. Paul, Head Analytical section for GPC, GC and SEM analysis reported in the present study. Mr. Manoj Kumar and Miss. Puja Kumari gratefully acknowledge the CSIR for awarding the Senior and Junior Research Fellowships respectively.",

year = "2011",

month = jan,

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doi = "10.1016/j.carbpol.2010.08.069",

language = "English",

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pages = "891--897",

journal = "Carbohydrate Polymers",

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T1 - An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

AU - Trivedi, Nitin

AU - Gupta, Vishal

AU - Kumar, Manoj

AU - Kumari, Puja

AU - Reddy, C. R.K.

AU - Jha, B.

N1 - Funding Information: The financial support received from CSIR (RSP 0016), New Delhi is gratefully acknowledged. We would also like to thank Dr. P. Paul, Head Analytical section for GPC, GC and SEM analysis reported in the present study. Mr. Manoj Kumar and Miss. Puja Kumari gratefully acknowledge the CSIR for awarding the Senior and Junior Research Fellowships respectively.

PY - 2011/1/10

Y1 - 2011/1/10

N2 - An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0-12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.

AB - An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0-12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.

KW - Cellulase

KW - Cellulose

KW - Halo-alkali tolerance

KW - Marine habitat

U2 - 10.1016/j.carbpol.2010.08.069

DO - 10.1016/j.carbpol.2010.08.069

M3 - Article

SN - 0144-8617

VL - 83

SP - 891

EP - 897

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 2

ER -

An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

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Keywords

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