TY - JOUR
T1 - Amperometric NADH determination via both direct and mediated electron transfer by NADH oxidase from Thermus aquaticus YT-1
AU - Somasundrum, Mithran
AU - Hall, Jennifer
AU - Bannister, Joe V.
PY - 1994/9/1
Y1 - 1994/9/1
N2 - Using NADH oxidase extracted from Thermus aquaticus YT-1, the determination of NADH by the direct oxidation of labile FADH2 and by mediated electron transfer using the Ru(III/IV) redox couple is described. A scheme for enzyme amplification is outlined using alcohol dehydrogenase (ADH) and NADH oxidase in solution, and enables 1–10 μM NADH to be determined. Detection is performed at + 150 mV using the RuCl62−/RuCl63− couple. Additionally, an electrode is modified by an ADH/NADH oxidase bilayer, enabling ethanol determination (20–200 μ M) at 0 mV via the oxidation of ruthenium red.
AB - Using NADH oxidase extracted from Thermus aquaticus YT-1, the determination of NADH by the direct oxidation of labile FADH2 and by mediated electron transfer using the Ru(III/IV) redox couple is described. A scheme for enzyme amplification is outlined using alcohol dehydrogenase (ADH) and NADH oxidase in solution, and enables 1–10 μM NADH to be determined. Detection is performed at + 150 mV using the RuCl62−/RuCl63− couple. Additionally, an electrode is modified by an ADH/NADH oxidase bilayer, enabling ethanol determination (20–200 μ M) at 0 mV via the oxidation of ruthenium red.
U2 - 10.1016/0003-2670(94)80333-1
DO - 10.1016/0003-2670(94)80333-1
M3 - Article
SN - 0003-2670
VL - 295
SP - 47
EP - 57
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1-2
ER -