Algiphilus aromaticivorans gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium isolated from a culture of the marine dinoflagellate Lingulodinium polyedrum, and proposal of Algiphilaceae fam. nov

Tony Gutierrez, David H Green, William B Whitman, Peter D Nichols, Kirk T Semple, Michael D Aitken

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A strictly aerobic, halotolerant, Gram-stain-negative, rod-shaped bacterium, designated strain DG1253(T), was isolated from a laboratory culture of the marine dinoflagellate Lingulodinium polyedrum (CCAP 1121/2). The strain was able to degrade two- and three-ring polycyclic aromatic hydrocarbons. It exhibited a narrow nutritional spectrum, preferring to utilize aliphatic and aromatic hydrocarbon compounds and small organic acids. Cells produced surface blebs and contained a single polar flagellum. The predominant isoprenoid quinone of strain DG1253(T) was Q-8. The fatty acid profile was dominated by C(18:1)¿7c. The mean DNA G+C content of strain DG1253(T) was 63.6 ± 0.25 mol%. 16S rRNA gene sequence analysis placed this organism within the order Xanthomonadales of the class Gammaproteobacteria. Its closest relatives included representatives of the Hydrocarboniphaga-Nevskia-Sinobacter clade (¿ 89.9% 16S rRNA gene sequence similarity) in the family Sinobacteraceae. On the basis of distinct phenotypic and genotypic characteristics, strain DG1253(T) is considered to represent a novel species of a new genus in the class Gammaproteobacteria, for which the name Algiphilus aromaticivorans gen. nov., sp. nov. is proposed. The type strain of the type species, Algiphilus aromaticivorans, is DG1253(T) (=ATCC BAA-2243(T)=DSM 24793(T)). In addition, a new family, Algiphilaceae fam. nov., is proposed to accommodate the genus Algiphilus.
Original languageEnglish
Pages (from-to)2743-2749
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume62
Issue number11
Early online date10 Jan 2012
DOIs
Publication statusPublished - Nov 2012

Keywords

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial
  • Dinoflagellida
  • Fatty Acids
  • Gammaproteobacteria
  • Molecular Sequence Data
  • Phylogeny
  • Polycyclic Hydrocarbons, Aromatic
  • Quinones
  • RNA, Ribosomal, 16S
  • Sequence Analysis, DNA

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