Absorption, disposition, metabolism, and excretion of [3- 14C]caffeic acid in rats

Maizatul H. Omar, William Mullen, Angelique Stalmach, Cyril Auger, Jean Max Rouanet, Pierre Louis Teissedre, Stuart T. Caldwell, Richard C. Hartley, Alan Crozier

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Male Sprague-Dawley rats ingested 140 ×106 dpm of [3- 14C]trans-caffeic acid, and over the ensuing 72 h period, body tissues, plasma, urine, and feces were collected and the overall levels of radioactivity determined. Where sufficient radioactivity had accumulated, samples were analyzed by HPLC with online radioactivity and tandem mass spectrometric detection. Nine labeled compounds were identified, the substrate and its cis isomer, 3′-O- and 4′-O-sulfates and glucuronides of caffeic acid, 4′-O-sulfates and glucuronides of ferulic acid, and isoferulic acid-4′-O-sulfate. Four unidentified metabolites were also detected. After passing down the gastrointestinal tract, the majority of the radiolabeled metabolites were excreted in urine with minimal accumulation in plasma. Only relatively small amounts of an unidentified 14C-labeled metabolite were expelled in feces. There was little or no accumulation of radioactivity in body tissues, including the brain. The overall recovery of radioactivity 72 h after ingestion of [3-14C]caffeic acid was ∼80% of intake.

Original languageEnglish
Pages (from-to)5205-5214
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number20
DOIs
Publication statusPublished - 23 May 2012

Keywords

  • [3-C]caffeic acid
  • absorption
  • disposition
  • excretion
  • metabolism
  • rats

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