Prostaglandin H2 synthase (PGHS) is the enzyme that catalyses the two-stage conversion of arachidonic acid to prostaglandin H2 (PGH2) prior to formation of prostanoids that are important in inflammation. PGHS isozymes (-1 and -2) are the target for nonsteroidal anti-inflammatory drugs (NSAIDs). Given the rekindled interest in specific anti-inflammatory PGHS inhibitors with reduced unwanted side effects, it is of paramount importance that there are reliable and efficient techniques to test new inhibitors. Here, we describe a novel in vitro electron paramagnetic resonance (EPR)-based assay for measuring the activity of PGHS-1.
Turnbull, C. M., McClure, D., Rossi, A. G., & Megson, I. L. (2006). A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity. Journal of Inflammation (London, England), 3, 12. https://doi.org/10.1186/1476-9255-3-12