A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity

Catriona M Turnbull, Danny McClure, Adriano G Rossi, Ian L Megson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Prostaglandin H2 synthase (PGHS) is the enzyme that catalyses the two-stage conversion of arachidonic acid to prostaglandin H2 (PGH2) prior to formation of prostanoids that are important in inflammation. PGHS isozymes (-1 and -2) are the target for nonsteroidal anti-inflammatory drugs (NSAIDs). Given the rekindled interest in specific anti-inflammatory PGHS inhibitors with reduced unwanted side effects, it is of paramount importance that there are reliable and efficient techniques to test new inhibitors. Here, we describe a novel in vitro electron paramagnetic resonance (EPR)-based assay for measuring the activity of PGHS-1.
Original languageEnglish
Pages (from-to)12
JournalJournal of Inflammation (London, England)
Volume3
DOIs
Publication statusPublished - 2006

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