A Method of Analysis of Isoprostanes in Salmon Tissue

Martina Bolechova; Josef Caslavsky; Daniela Macova; Milada Vavrova; Mark Taggart

A Method of Analysis of Isoprostanes in Salmon Tissue

Translated title of the contribution: METODA PRO ANALÝZU ISOPROSTAN Ů VE TKÁNI LOSOSA

Martina Bolechova, Josef Caslavsky, Daniela Macova, Milada Vavrova, Mark Taggart

Environmental Research Institute

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Abstract

A sensitive and reliable method is proposed allowing quantification of isoprostanes in salmon tissue using ultra-HPLC coupled to tandem MS. Solid phase extraction is used for sample concentration and purification. Repeatability, linearity, limits of detection and quantification were assessed by validation.

Translated title of the contribution	METODA PRO ANALÝZU ISOPROSTAN Ů VE TKÁNI LOSOSA
Original language	Other
Pages (from-to)	305-312
Number of pages	7
Journal	Chemicke Listy
Volume	109
Issue number	4
Publication status	Published - Apr 2015

Keywords

oxidative stress
mass spectrometry
liquid chromatography
isoprostanes

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title = "A Method of Analysis of Isoprostanes in Salmon Tissue",

abstract = "A sensitive and reliable method is proposed allowing quantification of isoprostanes in salmon tissue using ultra-HPLC coupled to tandem MS. Solid phase extraction is used for sample concentration and purification. Repeatability, linearity, limits of detection and quantification were assessed by validation. ",

keywords = "oxidative stress, mass spectrometry, liquid chromatography, isoprostanes",

author = "Martina Bolechova and Josef Caslavsky and Daniela Macova and Milada Vavrova and Mark Taggart",

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AU - Bolechova, Martina

AU - Caslavsky, Josef

AU - Macova, Daniela

AU - Vavrova, Milada

AU - Taggart, Mark

PY - 2015/4

Y1 - 2015/4

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AB - A sensitive and reliable method is proposed allowing quantification of isoprostanes in salmon tissue using ultra-HPLC coupled to tandem MS. Solid phase extraction is used for sample concentration and purification. Repeatability, linearity, limits of detection and quantification were assessed by validation.

KW - oxidative stress

KW - mass spectrometry

KW - liquid chromatography

KW - isoprostanes

UR - http://www.chemicke-listy.cz/common/article-vol_109-issue_4-page_305.html

M3 - Article

SN - 0009-2770

VL - 109

SP - 305

EP - 312

JO - Chemicke Listy

JF - Chemicke Listy

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ER -

A Method of Analysis of Isoprostanes in Salmon Tissue

Abstract

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